形觉剥夺下雏鸡视网膜、脉络膜和巩膜中全反视黄酸含量的变化  

Change of all-trans retinoic acid content in the retina,choroid and sclera of the chick with different visual environment

作　　者：许军[1] 赵江月[1] 王洪学[2] 孙林[3] 徐艳春[4] 张劲松[1] 

机构地区：[1]中国医科大学附属第四医院眼科,中国辽宁省沈阳市110005 [2]中国吉林省白山市八道江区医院眼科,134300 [3]中国医科大学基础医学院化学教研室,中国辽宁省沈阳市110001 [4]中国医科大学附属第一医院眼科,中国辽宁省沈阳市110001

出　　处：《国际眼科杂志》2007年第3期677-681,共5页International Eye Science

摘　　要：目的:雏鸡形觉剥夺性近视眼及形觉剥夺性近视恢复眼中视网膜、脉络膜和巩膜中视黄酸含量的作用。方法:选用新孵出的普通肉食家鸡75只,采用半透明薄膜眼罩遮盖的方法对左眼进行形觉剥夺,分为形觉剥夺组,遮盖时间为14d;形觉剥夺恢复组,遮盖11d后,去遮盖3d。两组的右眼作为对照眼。暗室内处死小鸡后,立即摘出眼球。冰台上用角膜钻取直径为8mm的后极部眼组织块,手术显微镜下快速分离出视网膜、脉络膜及巩膜纤维层和软骨层组织。将每3个标本作为一个样品。取空白样品,加入全反视黄酸标准溶液,按样品处理方法操作后,绘制标准曲线,根据标准品的色谱分析,分别确定出各组织中视黄酸的出峰位置,根据计算机的色谱工作站计算出样品的含量。结果:正常眼视网膜、脉络膜、巩膜中均有RA存在,其中脉络膜中RA含量最高,其次为巩膜及视网膜,其中巩膜纤维层的含量高于软骨层中的含量(P<0.05)。形觉剥夺14d后,视黄酸含量在视网膜中明显增高(P<0.01,n=10);在脉络膜中明显下降(P<0.01);在巩膜软骨层及纤维层中RA均明显下降(P<0.01),其中,纤维层中下降得更为明显。除去形觉剥夺3d后,视黄酸含量在视网膜中明显下降(P<0.01);在脉络膜中明显升高(P<0.01),为剥夺眼视黄酸水平的7倍;在巩膜软骨层和纤维层中明显升高(P<0.01),且纤维层中的含量高于软骨层中的含量(P<0.05)。结论:形觉剥夺及去形觉剥夺时,小鸡视网膜、脉络膜、巩膜纤维层及软骨层中视黄酸含量均发生了明显的变化,后极部巩膜纤维层中视黄酸含量变化比软骨层更为明显。AIM： To study the role of the all-bans retinoic acid （RA） level of retina, choroid and sclera in chicken form-deprivation myopic eyes and form-deprivation myopic recovery eyes. METHODS： Seventy-five newly incubated carnivorous chicken were selected. Their left eyes were covered with semitransparent membrane to deprive form perception, and were divided into 2 trial groups： form-deprived group： covering for 14 days, and recovery group： covering for 11 days followed by uncovering for 3 days. Unoccluded eye in either group served as control eyes respectively. Eyeballs were extracted immediately after the chickens were executed at the dark room. Posterior ocular tissue of 8mm in diameter was taken by cornea drill on dry ice table. Retina, choroid and sclera fiber layer and cartilage layer were isolated rapidly under microscope. Every 3 specimen were treated as one sample. Standard solution of all-trans retinoic acid was added into blank sample and after sample processing procedures, standard curves were protracted. The peak position of retinoic add in all tissues was found according to chromatography analysis of control standard substance. Rnally the levels of retinoic acid in samples were calculated by computer chromatography workstation. RESULTS： In normal eyes, RA existed in retina, choroids and sclera, with choroids the most followed by sclera and retina, with fibrous layer contained more than cartilage layer （P〈0.05）. After 14 days of form-deprivation, RA in retina increased significantly （P 〈0.01, n =10）, while decreased significantly in choroids, sclera cartilage layer and fibrous layer （P〈0.01）, among which scleral cartilage layer deceased the most. After 3 days recovery from form-deprivation, RA in retina decreased significantly （P 〈0.01）, while increased significantly in choroids, which was 7 times greater than form-deprivation eyes. RA level in sclera cartilage layer and fibrous layer elevated significantly （P 〈0.01）, with scleral fibrous layer contained mo

关 键 词：全反视黄酸 幼鸡 形觉剥夺 近视 巩膜 视网膜 脉络膜 高效液相色谱 

分 类 号：R778.11[医药卫生—眼科]