培养鼠视皮层神经元γ-氨基丁酸激活电流的特点  

Electrophysiological characteristics of GABA-activated currents in vitro cultured visual cortex neurons from rats

作　　者：张荻[1] 王美迟[2] 吴小影[1] 刘双珍[1] 

机构地区：[1]中南大学湘雅医院眼科,中国湖南省长沙市410008 [2]湖南师范大学生命科学学院,中国湖南省长沙市410081

出　　处：《国际眼科杂志》2007年第3期701-702,共2页International Eye Science

基　　金：中国湖南省自然科学基金资助项目(No.06JJ4099)~~

摘　　要：目的:研究体外培养大鼠视皮层神经元GABA激活电流的特点。方法:用膜片钳记录仪采用全细胞模式记录体外培养大鼠视皮层神经元GABA激活电流。结果:大部分受检细胞可记录到明显去敏感的内向电流,且具有剂量依赖性。IGABA可被足量bicuculline完全阻断。GABAA受体的失敏恢复时间为5min。结论:体外培养视皮层神经元GABA激活电流的特点为探讨视觉形成及其发育机制提供了电生理学依据。AIM： To study the electrophysiological characteristics of gamma-aminobutyric acid （GABA）-activated currents in vitro cultured visual cortex neurons from rats. METHODS： The patch clamp was used to record the whole cell currents of GABA-A channels in vitro cultured visual cortex neurons of 11-15d from the neonatal rats. RESULTS： It was found that majority of the examined neurons were sensitive to GABA. GABA could induce an inward current which was dose dependent. GABA-activated currents could be blocked completely by sufficient bicuculline. The recovery time of GABA-A receptors from desensitization was 5 minutes. CONCLUSION： The study of characteristics of GABA- activated currents provides electrophysiological evidence for the formation and developmental mechanism of vision.

关 键 词：视皮层γ-氨基丁酸 膜片钳 

分 类 号：R77[医药卫生—眼科]