家蚕iap基因在原核表达系统中的表达  

Expression of iap Gene of Bombyx mori in Prokaryotic Organism

作　　者：李西波[1] 陈劲频[1] 吴岩[1] 王文兵[1] 

机构地区：[1]江苏大学生命科学研究院

出　　处：《河南农业科学》2007年第5期47-49,53,共4页Journal of Henan Agricultural Sciences

基　　金：国家重点基础研究发展计划"973"项目(2005CB121005);江苏省教育厅高校科学研究项目(02KJD18003);江苏大学自然科学创新预研基金项目(04CX08)

摘　　要：为研究IAP蛋白抑制细胞凋亡的作用,用PCR法扩增出家蚕iap基因的cDNA,然后插入pET28a载体,得到阳性质粒pET28a-iap,并转化感受态细胞BL21,使用异丙基硫代-β-D-半乳糖苷(IPTG)诱导IAP蛋白的表达,应用SDS-PAGE和Western Blotting法分析目的蛋白,得到一条分子量约为38.8 kDa的特异表达条带,与表达产物的理论值相符。为进一步研究BmIAP蛋白的功能奠定了基础。To investigate the effects of the Bombyx mori IAP protein on anti-apoptosis, the lap gene was cloned by RT-PCR and was sequenced. The lap gene was then integrated into the plasmid PET28a. The recombinant plasmid was transformed into E. coli BL21. Expression of E. coli BL21 （DE3） was induced by isopropylthio- β- D- galactoside （IPTG）. The product was analyzed by SDS-PAGE and Western Blotting. The result showed that a specific band about 38.8 kDa was obtained, indicating that the lap gene of Bombyx mori was highly expressed.

关 键 词：家蚕 IAP蛋白 凋亡 原核表达 

分 类 号：S881.2[农业科学—特种经济动物饲养]