ACA微囊化牛嗜铬细胞小鼠腹腔移植热板镇痛实验  

The analgesic effect of ACA encapsulated bovine chromaffin cells intraperitoneally transplanted in mice is illustrated by hot plate test

作　　者：郭慧琴[1] 雄鹰[2] 程谦涛[1] 王为[3] 马小军[3] 

机构地区：[1]淮安市第二人民医院神经内科,江苏淮安223002 [2]解放军第二一零医院神经内科,辽宁大连116027 [3]中国科学院大连化学物理研究所医用材料工程组,辽宁大连116023

出　　处：《徐州医学院学报》2007年第6期362-364,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30472102);大连市科技计划项目(2004B3SFF161)

摘　　要：目的探讨海藻酸钠-壳聚糖-海藻酸钠(ACA)微囊化牛嗜铬细胞移植到小鼠腹腔后的镇痛效应。方法采用静电液滴法制备ACA微囊化的牛嗜铬细胞,并将其移植到小鼠腹腔,设海藻酸钠-聚赖氨酸-海藻酸钠(APA)微囊化的牛嗜铬细胞、未微囊化的牛嗜铬细胞、空的ACA微胶囊和生理盐水组进行对比。通过小鼠热板镇痛试验来考察ACA微囊化牛嗜铬细胞的镇痛作用。结果ACA微囊化牛嗜铬细胞小鼠腹腔移植后可以使小鼠热板痛阈延长,有效镇痛时间长达1个月,痛阈延长时间较APA微胶囊化牛嗜铬细胞长;未微囊化的牛嗜铬细胞无明显的镇痛作用(P>0.05)。结论移植到小鼠腹腔中的ACA微囊化的牛嗜铬细胞可以起到镇痛作用,其有效镇痛时间较APA微囊化的牛嗜铬细胞长。Objective To examine the analgesic effect of alginate - chitosan - alginate （ ACA ） microencapsulated bovine chromaffin cells （BCC） transplanted in the peritoneal cavity in mice. Methods Electrostatic droplet technique was used to prepare the microencapsulated bovine chromaffin cells. ACA microencapsulated bovine chromaffin cells （ ACA - BCC ） were transplanted to the peritoneal cavity of mice to form the test group. The role of control was played by the mice with transplanted alginate - polylysine - alginate microencapsulated bovine chromaffin cells （ APA - BCC group）, unencapsulated bovine chromaffin cells （BCC group）, empty ACA microcapsules （ACA group） and normal saline （ NS group）. Hot plate test was adopted to evaluate the analgesia effect of microencapsulated BCC. ResultsACA - BCC prolonged the latency in hot plate test; the analgesia lasted for more than 1 month, which appeared earlier and was longer than that in the APA - BCC group. The unencapsulated BCC had no significant analgesic effect. Conclusion ACA - BCC can induce analgesia when they are transplanted in the peritoneal cavity of mice. The immunoprotective effect of ACA microcapsule is better than that of APA one. The effective analgesic time of ACA - BCC is longer than that of APA - BCC.

关 键 词：壳聚糖 海藻酸钠 微胶囊 嗜铬细胞 移植 镇痛 热板试验 小鼠 

分 类 号：R318.08[医药卫生—生物医学工程]