大口径毛细管气相色谱法直接测定复方阿司匹林制剂  

Direct Determination of Aspirin Combined Formulation by Gas Chromatography with Wide Bore Capillary Column

作　　者：姚如心[1] 许庆琴[1] 杜黎明[1] 

机构地区：[1]山西师范大学分析测试中心,山西临汾041004

出　　处：《分析科学学报》2007年第3期295-298,共4页Journal of Analytical Science

基　　金：山西省自然科学基金(No.20041030)

摘　　要：建立了同时测定复方阿司匹林制剂中阿司匹林、对乙酰氨基酚、咖啡因及降解产物水杨酸的大口径毛细管气相色谱法.在HP-1大口径色谱柱上直接进样,无需衍生化处理,所测组分与内标物2 min内达到基线分离.各组分在其线性范围内线性关系良好（相关系数均高于0.999）,检出限分别为10.0 μg/L、5.0μg/L、1.0μg/L和1.0μg/L.经样品测定,平均回收率为97.46%～101.24%.该法简便、快速、准确、重现性好,可用于复方阿司匹林制剂的质量控制.An accurate, simple, reproducible and sensitive method for direct determination of aspirin （AS）, paracetamol （PA）, caffeine （CA） and salicylic acid （SA） in combined formulation by gas chromatography without any derivation has been developed. The separation of several components was achieved on wide bore capillary column HP-1 （5 m× 0. 53 mm×2. 65μm） with n-cetane as internal standard and programmed column temperature from 180℃ to 230℃ at 8℃/min, holding 2 min. The flame ionization detector （FID） was used as detector. The linear ranges of detection for AS, PA, CA andSA were 0. 25-20. 0 mg/L, 0. 125-10. 0 mg/L,0. 01-4. 0 mg/L and 0. 01-4.0 mg/L, respectively. The detection limits were 10, 5. 0, 1. 0 and 1. 0 μg/L, respectively, and the average recoveries were 98.10%-101.24%. The method has been applied successfully to the determination of fourfold mixture of AS, PA,CA and SA in pharmaceutical preparations. There was no interference from drugs administered with AS,PA,CA and SA.

关 键 词：大口径毛细管气相色谱法 阿司匹林 对乙酰氨基酚 咖啡因 水杨酸 

分 类 号：O657.71[理学—分析化学] O658.2[理学—化学]