离体脑肿瘤高分辨魔角旋转磁共振质子波谱  

To Explore In Vitro Brain Tumors with High Resolution Magic Angle Spinning Hydrogen Magnetic Resonance Spectroscopy in Vitro

作　　者：谭伟[1] 柯要军[1] 简纪华[1] 

机构地区：[1]武汉科技大学附属医院介入放射科,武汉430064

出　　处：《放射学实践》2007年第6期550-553,共4页Radiologic Practice

摘　　要：目的：利用高分辨魔角旋转质子磁共振波谱（HRMAS-^1H MRS）检测脑肿瘤手术标本,分析胶质瘤及脑膜瘤水溶性代谢物特征,探讨HRMAS-^1H MRS临床应用价值。方法：30例病理证实脑肿瘤,6例Ⅰ-Ⅱ级星形细胞瘤,7例间变（Ⅲ级）星形细胞瘤,10例胶质母细胞瘤（Ⅳ级）和7例脑膜瘤。Varian公司600MHz谱仪上Nano探头采集HRMAS-^1H MRS,jMRUI3.2版软件处理,总结代谢物出现几率,比较代谢物与Cr比值。结果：HRMAS-^1H MRS能分辨NAA、PCr/Cr、GPC＋PCho＋Cho、Glu/Gln、Gly、Tau、Ala、Lac、mI等脑内多种代谢物。所有样本均显示Lac峰,6例样本在3.72ppm或3.90ppm处显示一组不知名的单峰。6例Ⅰ-Ⅱ级星形细胞瘤Cho/Cr2.42±1.01,与胶质母细胞瘤组比较P=0.003;7例间变性星形细胞瘤Cho/Cr3.48±0.59,与胶质母细胞瘤组比较P=0.01;10例胶质母细胞瘤Cho/Cr变化范围大,从0.9-11.3,平均值5.40±1.23。从Ⅰ-Ⅱ级星形细胞瘤到胶质母细胞瘤Gly、Tau、mI、Ala出现几率增加,Ala/Cr、Tau/Cr和Gly/Cr有升高趋势;胶质瘤Ala/Cr0.31±0.13。脑膜瘤Ala峰和Cho峰较高,Cr峰较胶质瘤下降。4/7例无NAA峰,3/7例有较低NAA峰。Cho/Cr3.56±1.01;Ala/Cr0.53±0.28,与胶质瘤比较P=0.006。结论：HRMAS-^1H MRS能进一步显示波谱细节,分辨活体波谱中Cho峰下代谢物,显示胶质瘤良恶性程度。随着恶性程度增加,Cho、mI、Ala、Tau、Gly增加。HRMAS-^1H MRS是唯一能得到病理标本各项同性波谱的方法。Objective：To detect specimens of brain tumors with HRMAS-^1H MRS,to analyze their soluble metabolites, and discuss clinical value about the technique. Methods： There were 30 cases of brain tumor confirmed by pathology, including Ⅰ-Ⅱ graded astrocytomas （n=6）,Ⅲ graded astrocytomas （n=7）,Ⅳ glioblastomas （n=10）,meningiomas （n= 7）. All data were got with Varian 600MHz spectrometer and analyzed on MRUI software. Results： HRMAS-^1H MRS could resolve lots of metabolites, such as NAA, PCr/Cr, GPC＋ PCho＋ Cho,Glu/Gln,Gly, Tau, Ala, Lac, mI, etc. All samples had Lac peak. 6 samples showed unknown peak at 3. 72ppm or 3. 90ppm. The ratio of Cho/Cr to Ⅰ-Ⅱ astrocytomas was 2.42±1.01,compared with glioblastomas （5.40±1.23） ,P=0. 003. The ratio of Cho/Cr to astrocytomas was 3.48±0.59, compared with glioblastomas,P=0.01. The showed opportunities of Gly, Tau, mI and Ala were improved with the upgrading of astrocytoma. The values of Ala/Cr,Tau/Cr and Gly/Cr were elevated. The ratio of Ala/Cr to gliomas was 0.31±0. 13.3. In meningiomas,4 cases showed no NAA,3 cases presented NAA. The ratio of Ala/Cr was 0. 53±0. 28,compared with gliomas, P 0. 006. Conclusions： HRMAS-^1H MRS can show more detail spectrum, resolve ingredients of the Cho corn pound and graded degree of gliomas. With the malignancy of gliomas raising, Cho, mI, Ala, Tau and Gly may elevate. HRMAS^-1H MRS is the only method which is able to acquire isotropic spectroscopy of solid samples.

关 键 词：磁共振波谱 离体 脑肿瘤 

分 类 号：R739.4[医药卫生—肿瘤]