三氧化二砷脂质体的制备及其对鼠脑胶质瘤的影响  

Preparation and effects of arsenic trioxide liposome on glioma cell apoptosis in rats

作　　者：张旭[1] 赵世光[1] 任颖 宋大勇[3] 赵洪波[1] 王慧博[1] 邹慧超[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001 [2]黑龙江省第二医院神经外科,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第四医院神经外科,黑龙江哈尔滨150001

出　　处：《中国微侵袭神经外科杂志》2007年第6期269-272,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金资助项目(30240043);黑龙江省重大攻关基金资助项目(GB05C401-01)

摘　　要：目的研究三氧化二砷(As2O3)脂质体注射液对大鼠体内C6胶质瘤细胞凋亡的影响。方法采用超声薄膜分散法制备As2O3脂质体,将126只成瘤大鼠分为As2O3脂质体组、As2O3组、生理盐水组。原子荧光法检测注射As2O3脂质体和As2O3后大鼠脑组织中As2O3浓度。从电镜、TUNNEL和大鼠生存时间等方面研究As2O3脂质体对C6胶质瘤的影响。结果As2O3脂质体提高了As2O3的血-脑屏障通过。电镜和TUNEL检测显示:As2O3脂质体组细胞凋亡率给药后3d为(13.53±1.68)%,7d为(20.03±0.79)%,多于As2O3组和盐水组。动物生存时间亦优于其他两组。结论超声薄膜分散法是制备As2O3脂质体的较好方法。As2O3脂质体可较As2O3更明显地诱导鼠脑胶质瘤细胞凋亡,延长载瘤鼠的生存期。Objective To develop the injection of liposome-encapsulated arsenic trioxide （As203 liposome） and investigate the effects of As203 liposome on C6 glioma cell apoptosis in tumor-bearing rats, Methods The liposomes were developed by ultrasonic wafer dispersion. The tumor-bearing rats （126 rats） were equally randomized into As203 liposome group, As203 group and saline group and injected intravenously with above 3 kinds of medicine respectively. As203 concentration was measured by using atomic fluorescence technique in As203 liposome group and As203 group. The effects of As203 liposomes on C6 glioma cells in the three groups were studied by electronic microscopy, TUNNEL staining and survival time, Results As203 liposomes obviously facilitated BBB penetration of As203. The electronic microscopic analysis and TUNNEL staining results showed tumor cell apoptosis rate was 13.53±1.68% on the 3rd day and 20.03±0.79% on the 7th day after drug injection in As203 liposome, much higher than those of As203 group and saline group, The survival time was also much longer in As203 liposome group than in the other two groups. Conclusion Ultrasonic wafer dispersion is a good method for developing As203 liposomes. The As203 liposomes can more significantly induce apoptosis ofglioma cells and prolong the life span of glioma-bearing rats than As203 alone.

关 键 词：砷剂 脂质体 神经胶质瘤 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]