能量可控陡脉冲抗肿瘤血管的作用  

An Experimental Study of Energy Controllable Steep Pulse in the Treatment of Rat with Subcutaneous Transplantive Tumor

作　　者：罗小东[1] 胡丽娜[2] 孙才新[3] 熊兰[3] 米彦[3] 

机构地区：[1]重庆医科大学第二附属医院妇产科,重庆400010 [2]四川大学华西第二医院妇产科,成都610041 [3]重庆大学高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2007年第3期492-495,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30271484);CMB资助项目;四川省科技厅攻关项目资助(05SG022-013-2)

摘　　要：建立Wistar大鼠皮下瘤模型,研究能量可控陡脉冲(Energy controllable steep pulse,ECSP)对荷瘤大鼠移植瘤的影响,免疫组化方法检测血管内皮生长因子、微血管密度的表达。实验结果观察到ECSP处理组肿瘤体积减小,血管内皮生长因子、微血管密度的表达均降低,且与对照组比较均具有显著性差异。ECSP处理后肿瘤生长明显受到抑制。This experimental study was designed to investigate the effects and the expressions of microvessel density （MVD）, vascular endothelial growth factor （VEGF） on the transplanted tumor in the rat model with Walker-256 after energy controllable steep pulse（ECSP）. The experiment revealed that the steep pulse electrical field has better effect on tumor, compared with the control. The positive cell staining intensity of VEGF in the control group was significantly higher than that in ECSP group （P〈0. 05）. The number of MVD in the tumor tissues of ECSP group was significantly lower than that of tumor control group（P〈0. 05）. These results showed that ECSP could inhibit the growth and angiogenesis of tumor and its pathway is to down-regulate the expression of VEGF possibly.

关 键 词：陡脉冲 血管 肿瘤 

分 类 号：R730.5[医药卫生—肿瘤]