聚焦超声结合原卟啉对S180肿瘤细胞杀伤作用的研究  

The Killing Effect of Focused Ultrsound Activating Protoporphyrin IX on S180 Cells

作　　者：任耀辉[1] 王筱冰[1] 王攀[1] 郝巧[1] 刘全宏[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《生物医学工程学杂志》2007年第3期626-630,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(39870240;30270383)

摘　　要：采用频率为2.2MHz及不同强度的聚焦超声激活原卟啉对小鼠腹水型S180肿瘤细胞的杀伤作用进行研究。台盼蓝拒染法检测了不同处理后细胞的相对存活率,通过扫描电镜和透射电镜观察细胞超微结构的变化。实验结果表明:单纯原卟啉对S180肿瘤细胞几乎没有影响,单纯超声和超声结合原卟啉对细胞均有一定的损伤,肿瘤细胞受损程度随着声照强度的增加而加剧,并且在一定范围内随着原卟啉剂量浓度的加大,细胞受损也逐渐增强。当原卟啉浓度为120μM时,超声激活原卟啉对腹水型S180肿瘤细胞的协同杀伤效应表现最为明显。The killing effect on S180 cells was studied using the combination of protoporphyrin IX and focused ultrasound at the frequency of 2.2 MHz and different intensities. Cell viability was determined by trypan blue exclusion test, morphology changes were evaluated by means of scanning electron microscopy and transmission electron microscopy after ultrasonic exposure. The results indicated that protoporphyrin IX （PPIX） alone showed no significant effect on S180 cells when compared with that of control group. Ultrasound alone and ultrasound combined with PPIX groups showed some anti-tumor effect , which became more noticeable as the ultrasound intensity and PPIX concentration increased, and when the concentration of PPIX increased to 120 /zM, the ultrasound combined with PPIX exerted a more significant antl-tumor effect than did the ultrasound alone in the same experiment.

关 键 词：超声 原卟啉 S180细胞 抗肿瘤效应 

分 类 号：R73-3[医药卫生—肿瘤]