H9N2亚型猪流感病毒A/Swine/Shandong/1/02分离株的基因序列分析  

Sequence analysis of genes of H9N2 subtype swine influenza virus isolate A/Swine/Shandong/1/02

作　　者：杨焕良[1] 乔传玲[1] 陈艳[1] 辛晓光[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处：《中国兽医科学》2007年第6期473-476,共4页Chinese Veterinary Science

基　　金：国家科技攻关计划项目(2004BA519A55)

摘　　要：从有肺炎症状的病猪中分离到1株H9N2亚型猪流感病毒（SIV）A／Swine／Shandong／1／02，对其进行了全病毒基因序列分析。结果表明，该毒株8个基因片段的核苷酸序列均来自禽流感病毒（AIV），与我国目前家禽中流行的H9N2亚型AIV毒株具有很高的同源性，与A／Duck／Hong Kong／Y280／97（H9N2）的同源性为94．1％～98．9％，与A／Chicken／Beijing／1／94（H9N2）的同源性为94．5％～98．2％；推导的其血凝素（HA）裂解位点处的氨基酸序列为P—A-R—S-S-R，完全符合H9亚型AIV欧亚分支中的类A／Chicken／Beijing／1／94亚分支的特征；基因分析结果表明，该分离株的所有基因片段均来源于H9N2亚型AIV，它可直接感染猪，并导致发病，但它并未在猪体内发生重组。Genetic characteristics of an influenza A（H9N2） virus, A/Swine/Shandong/1/02, isolated in Shandong Province from a pig with pneumonia, was analysed. The virus was proved to be genetically similar to the avian influenza A（H9N2） viruses currently circulating in Chinese poultry. Eight gene segments of A/Swine/Shandong/1/02（H9N2） were closely related to those of A/Duck/Hong Kong/Y280/97 （H9N2） and A/Chicken/Beijing/1/94 （H9N2）, i.e. the identity rates of the gene segments between A/Swine/Shandong/1/02 and A/Duck/Hong Kong/Y280/97 or A/Chicken/Beijing/1/94 were 94. 1%- 98.9% or 94. 5%- 98. 2%, respectively. The deduced amino acid sequence of HA cleavage site from A/Swine/Shandong/1/02 was P-A-R-S-S-R, which was accordance with characteristics of A/Chicken/ Beijing/1/94 in the Eurasian branch of H9 subtype avian influenza virus（AIV）. It was concluded that all the gene segments of A/Swine/Shandong/1/02 were from H9N2 AIV, and it could infect swine directly, and cause disease, but did not recombine in swine body.

关 键 词：猪流感病毒 H9N2 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q523.8[农业科学—兽医学]