产碱菌麦芽四糖淀粉酶多型性的形成  

Studies on the Multiple forms of Maltotetraose forming Amylase from Alcaligenes sp.

作　　者：朱静[1] 车凤琴[1] 严自正[1] 梁改芹[1] 张树政[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》1997年第1期24-28,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金;中国科学院资助

摘　　要：产碱菌培养液的酶谱呈一主带Ｇ４Ａ１及两次带Ｇ４Ａ２与Ｇ４Ａ３，分别进行纯化并鉴定其水解产物，均为麦芽四糖，说明三者全是麦芽四糖淀粉酶，它们以多型性形式存在于产碱菌培养液中。培养液中没有检出糖苷酶，２４，７２，９６ｈ培养液中不含蛋白酶（仅４８ｈ培养液中有微量蛋白酶），说明多型性不是糖苷酶和蛋白酶造成的。改变培养条件不影响多型性产生。Zymograms of the cultural supernatant of Alcaligenes sp. showed three bands,the major one G 4 A 1,the minor two,G 2 A 2 and G 4 A 3.Based on either the electrophoretic homogeneity of the purified three bands or the enzymatic activities identified by thin layer chromatogram of the soluble starch hydrolysates,all the three bands were confirmed to be maltotetraose forming amylase but in multiple forms.Neither glycosidase nor protease activities could be detected in culture (only a very weak protease activity were observed at 48 hrs after cultivation),which indicate that the amylase multiple form formation is not involved in these two enzymes.Only the relative amount of the two minor bands but not the multiple form pattern could be changed,if the cultivation conditions are prepared at the pH from 6 5～8 5,or with 0 3% glucose.

关 键 词：产碱菌 麦芽四糖淀粉酶 多型性 

分 类 号：Q939.106[生物学—微生物学]