P16^(ink4)cDNA的克隆及序列分析  

Molecular Cloning and Sequencing of P16 ink4 cDNA from Hela Cell

作　　者：黄长晖[1,2] 邓炜[1,2] 傅继梁[1,2] 

机构地区：[1]第二军医大学生物教研室医学分子遗传学开放实验室 [2]复旦大学遗传学研究所

出　　处：《生物工程学报》1997年第1期105-107,共3页Chinese Journal of Biotechnology

摘　　要：Ｐ１６ｉｎｋ４ｃＤＮＡ的克隆及序列分析黄长晖邓炜傅继梁（第二军医大学生物教研室，医学分子遗传学开放实验室上海２００４３３）（复旦大学遗传学研究所上海２００４３３）Ｐ１６ｉｎｋ４是ＣＤＫ（ＣｙｃｌｉｎＤｅｐｅｎｄｅｎｔＫｉｎａｓｅ）抑制蛋白家族中的...The P16 ink4 gene has many features of a tumor suppressor gene.To examine its function,P16 ink4 cDNA was amplified from the total RNA of Hela cell by reverse transcription PCR with the use of the primers 5′ GGGAATTCATGGAGCCGGCGGCGGGGA 3′ and 5′ GGGGATCCGGCCCTGTAGGACCTTCGG 3′.The PCR product including 471 bp open reading frame and 3′ 69 bp non coding rogion was cloned into M13mp18 and M13mp19 vector and verified by single stranded DNA sequencing.The scquence is indentical to the published sequence,that means P16 ink4 cDNA had been cloned.

关 键 词：基因克隆 P16^ink4基因 CDNA 序列分析 

分 类 号：Q785[生物学—分子生物学]