高效液相色谱法测定痹通药酒中阿魏酸的含量  

Determination of Ferulic Acid in Bitong Yaojiu by HPLC

作　　者：张翠英[1] 李振国[1] 马晓峰[2] 徐金玲[1] 刘乃强[1] 

机构地区：[1]河南省食品药品检验所,河南郑州450003 [2]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2007年第6期9-10,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立痹通药酒中阿魏酸的含量测定方法。方法:C18柱,以乙腈-0.1%磷酸(20∶80)为流动相,检测波长为316 nm。结果:阿魏酸的线性范围为4.072～101.8μg/mL(r=0.99996),平均回收率为99.31%,RSD为3.66%(n=6)。结论:该方法操作简便准确,重复性好,能有效控制痹通药酒的质量。Objective：To develop and HPLC method for determination of ferulic acid in Bitong Yaojiu. Methods： Analysis was carried out on a C18 （150 mm × 4.6 mm, 5 μm）column eluled with, acetonitrile-0.1% phosphoric acid buffer （20：80）as the mobile phase with UV detection at 316 nm. Results：The linear relationship of ferulic acid was in the range of 4. 072 - 101.8 μg/mL, the recovery was 99.31% （RSD 3.66 %, n = 6）. Conclusion： This method is simple, accurate and reproducible.

关 键 词：痹通药酒 阿魏酸 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]