受精前后地鼠卵母细胞皮质颗粒的变化  

Changes of Cortical Granules in Hamster Zona-free Oocyte before and after Fertilization

作　　者：江澍[1] 江一平[2] 

机构地区：[1]福建中医学院中西医结合系,福建福州350108 [2]福建医科大学细胞与发育工程研究中心,福建福州350004

出　　处：《福建中医学院学报》2007年第3期51-53,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助项目(Z0516042);福建省教育厅科技项目(JA03124)

摘　　要：为观察人精子与地鼠卵异体受精前后,卵母细胞内皮质颗粒的变化,采用罗丹明偶联的兵豆凝集素标记受精前、受精30min和3h后金黄地鼠的去透明带卵母细胞,用激光共聚焦显微镜观察皮质颗粒的分布情况,结果显示,受精前卵母细胞内的皮质颗粒散在于卵质膜下和皮质区,受精后皮质颗粒数量减少,并发生边集。说明人精子与金黄地鼠卵异体受精时,可引起皮质颗粒向卵质膜靠拢并胞吐。In order to observe the changes of cortical granules in hamster oocyte before and after fertilization, the assay of heterologous in vitro were done between human spermatozoa and hamster zona-free oocytes. The cortical granules were dyed with TRITC-LCA and were surveyed by the laser confocal microscope. The results showed that the cortical granules spreaded under the oolemma and in the cortex before fertilization, and the quantity of the cortical granules reduced after fertilization. These indicate that the cortical granules can close to the oolemma and exocytosis when human spermatozoa penetrate into hamster zona-free oocytes.

关 键 词：受精 皮质颗粒 胞吐 激光共聚焦 

分 类 号：R714.8[医药卫生—妇产科学]