含硒化合物联合医用长春新碱应用对艾氏腹水癌的抑制机制  

Inhibitory effect of selenium compounds combined with vincristine on Ehrlich ascites carcinoma and the mechanism of action

作　　者：宋筱瑜[1] 林德贵[1] 王雷[2] 王金秋[1] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]美国明尼苏达大学癌症研究中心,明尼阿波利斯55455

出　　处：《中国农业大学学报》2007年第3期14-18,共5页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30471275)

摘　　要：为寻找医治艾氏腹水癌的新型制剂,并评价药效,对小鼠腹腔注射肿瘤细胞,建立荷艾氏腹水癌细胞(EAC)的小鼠模型;并于接种后第7天开始用抗癌药,观察荷瘤小鼠(接种EAC后产生血性腹水的小鼠)的生存周期,通过对动物体内抗氧化水平指标(SOD、GSH、MDA)的检测,判定硒化合物的保护作用;用流式细胞术及对细胞进行DNA梯检测,评估药物对肿瘤细胞调亡的影响。结果表明含硒化合物,尤其是有机硒化合物,甲基硒代半胱氨酸(SeMC)与抗癌药长春新碱联合应用可使荷瘤小鼠的生存期延长、机体的抗氧化水平升高及肿瘤细胞凋亡率增多,较对照组有显著差异(P<0.01)。研究认为,含硒化合物与医用长春新碱联用对荷瘤小鼠的保护性,主要是出于硒的抗氧化能力,以及使长春新碱促进肿瘤细胞的凋亡来实现的。This study was designed to assess the effect and mechanism of action of selenium compounds and vincristine （VCR） on mice bearing Ehrlich ascites carcinoma （EAC）, a tumor-bearing mice, and compare the toxicity of a single drug with combination of two drugs. An EAC mouse model was established by injecting tumor cells intraperitoneally and drugs were given to mice on the 7th day after injection. The survival time of Ehrlich ascites tumor-bearing mice was recorded. The protective effect of selenium compounds on mice carrying tumor was evaluated by antioxidant indices （SOD, GSH, MDA） and their effects on tumor cells apoptosis evaluated by flow cytometry and DNA ladder. Selenium compounds, especially methylselenocysteine （MSeC） combined with VCR significantly （P〈 0.01） prolonged the life span of tumor-bearing mice, improved the antioxidant level in vivo, and simultaneously promoted the apoptosis of tumor cells. In general, selenium compounds combined with VCR played a powerful role in protection of tumor-bearing mice. This mainly depended on the anti-oxidant function of selenium but the anticancer activity of VCR was also enhanced in combination with selenium, by inducing apoptosis.

关 键 词：硒 长春新碱 小鼠模型 艾氏腹水癌 联合用药 

分 类 号：R73-36[医药卫生—肿瘤]