慢性情绪应激对大鼠卵巢功能影响的初步观察  

Ovarian function in rat model of chronic emotional stress

作　　者：程湘[1] 徐辉[2] 艾国平[2] 粟永萍[2] 谢荣凯[1] 陈正琼[1] 吴家燕[1] 

机构地区：[1]第三军医大学新桥医院妇产科,重庆400037 [2]第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2007年第14期1368-1370,共3页Journal of Third Military Medical University

基　　金：创伤;烧伤与复合伤国家重点实验室开放课题(2004)~~

摘　　要：目的观察慢性情绪应激对大鼠卵巢功能的影响。方法以不确定性空瓶饮水刺激作为情绪应激源,考察20d的慢性情绪应激对雌性大鼠行为、脾脏指数、动情周期和血中FSH、LH、E2和P含量的影响。结果慢性情绪应激明显诱发雌性大鼠的攻击行为,增加脾脏指数,差异非常显著(P<0.01)。慢性情绪应激延长雌性大鼠的动情周期,差异非常显著(P<0.01),但对卵巢指数没有显著影响(P>0.05)。慢性情绪应激减少雌性大鼠血中E2含量,差异非常显著(P<0.01),而对血中P含量没有显著影响(P>0.05);同时慢性情绪应激后大鼠血中FSH和LH含量下降,差异非常显著(P<0.01)。结论慢性情绪应激影响大鼠卵巢分泌功能,该模型可用于情绪应激对生殖内分泌影响的研究。Objective To observe the effect of chronic emotional stress on ovarian function in rats. Methods The uncertain empty bottle stress was used as a source of emotional stress, and 20 day stress was carried out in 20 female SD rats aged 3 months. Another 20 rats were used as controls. The behavior changes and estrous cycle were observed. The serum levels of FSH, LH, E2 and P were determined. Results The chronic emotional stress could increase attacking behavior and splenic index significantly （P 〈 0. 01 ）, and pro- long the estrous cycle significantly （P 〈0. 01 ）, but had no influence on ovarian index （P 〉0. 05）. It could decrease serum FSH, LH and E2 levels significantly （ P 〈 0. 01 ） , but had no effect on P levels （ P 〉 0. 05 ）. Conclusion The chronic emotional stress can influence the ovarian endocrine secretion in rats. This model may be used to study the influence of chronic emotional stress on generative endocrine secretion.

关 键 词：情绪应激模型 卵巢 性激素 动情周期 

分 类 号：R339.22[医药卫生—人体生理学] R395.1[医药卫生—基础医学]