复合PRP的可注射型组织工程骨修复兔桡骨缺损的实验研究  

Injectable tissue-engineered bone made of autologous platelet-rich plasma,fibrin glue and BMSCs for repairing segmental radial defect in rabbits

作　　者：黄爱文[1] 金丹[1] 裴国献[1] 曾宪利[1] 胡稷杰[1] 林海宁[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广州510515

出　　处：《第三军医大学学报》2007年第14期1374-1377,共4页Journal of Third Military Medical University

基　　金：国家高技术发展计划("863"计划)重大专项课题(2003AA205010)~~

摘　　要：目的以自体富血小板血浆（platelet-rich plasma,PRP）、骨髓基质干细胞（bone marrow stromal cells,BMSCs）和纤维蛋白胶构建可注射型组织工程骨并探讨其修复骨缺损的效果。方法36只新西兰兔分为A、B、C3组,每组12只。A、B组动物术前4h抽取耳背中央动脉血提取PRP。A组于术前1-2个月抽取双髂骨处骨髓并培养出BMSCs,在体外与纤维蛋白胶（FG）及自体PRP构建成可注型组织工程骨,植入自体桡骨1.5cm节段性骨缺损,为实验组。B、C两组分别植入PRP＋FG、FG于同样骨缺损处为对照组。另取4只桡骨同样部位和大小骨缺损旷置作为空白组。术后观察其一般情况并于4、8、12周取材做组织学切片,术后12周取尺桡骨做生物力学测试。分别从组织学观察、生物力学方面评估比较骨缺损的修复情况。结果组织学观察见A、B组各时间点新骨形成均优于C组。生物力学比较：12周时A组桡骨生物力学强度与正常桡骨比较无明显差异（P〉0.05）,但其明显优于B组（P〈0.05）。结论PRP对骨缺损愈合有明显促进作用,复合PRP的可注射型骨修复材料及构建的含种子细胞的组织工程骨均可修复节段性骨缺损,但种子细胞的添加可明显促进新骨成熟度和增强其生物力学性能。Objective To construct injectable tissue-engineered bone graft with fibrin glue （FG）, autologous platelet-rich plasma （PRP） and BMSCs and investigate its effect on repairing segmental radial defect in rabbits. Methods Thirty-six New-Zealand rabbits were randomly divided into 3 groups （n = 12 in each group）. A 1.5 cm segmental defect in right radius was made in each animal and three different bone grafts were implanted there respectively. Group A： FG ＋ PRP ＋ BMSCs, Group B： FG ＋ PRP, Group C： FG. About a month before operation, BMSCs withdrawn from bilateral iliac bones of rabbits were cultured and multiplied in vitro. Four hours before operation, PRP was extracted from autologous arterial blood and stored at - 70 ℃. Another 4 rabbits of which the same defects in radius left empty served as the blank group. At the end of 4, 8, 12 weeks after implantation, two rabbits were sacrificed and specimens were taken from the part of bone defect for histological＇examination. At the end of 12 weeks, all animals were sacrificed and the radius were taken to analyze the biomechanics strength. Results In groups A and B, most FG was absorbed and substituted by newly formed cortical bone in postoperative 8 weeks. Twelve weeks later, the mature new bone with plenty of bone matrix filled in the bone defect and there formed a medullary cavity in group A. The biomechanical strength of radius in group A showed no significant statistical difference with that of normal radius （ P 〉 0. 05 ）, but was better than that in group B （P 〈 0. 05 ）. Conclusion Since PRP can promote healing of bone defects, the injectable bone grafts made of autologous PRP, with or without BMSCs, are effective in repairing segmental bone defect, and the presence of BMSCs could improve the new bone maturation and increase the biomechanic strength.

关 键 词：富血小板血浆 组织工程骨 骨缺损 纤维蛋白胶 骨髓基质干细胞 

分 类 号：R-33[医药卫生] R318.1