HPLC-DAD同时测定两种内酯式他汀及有关物质  

HPLC-DAD determination of two lactone form statins and their related substances

作　　者：冯建立[1] 王学军[1] 许振良[1] 杨座国[1] 邴乃慈[1] 韦锦萍 

机构地区：[1]华东理工大学化学工程研究所膜科学与工程研发中心,上海200237 [2]上海卫康光学有限公司研发部,上海200126

出　　处：《食品科技》2007年第6期220-223,共4页Food Science and Technology

摘　　要：建立了高效液相色谱-二极管阵列检测法研究内酯式他汀的水解行为,同时测定洛伐他汀和辛伐他汀及有关物质。色谱条件为:Waters Symmetry C18色谱柱(4.6mm×150mm,5μm);乙腈-磷酸二氢钾缓冲液(60∶40)为流动相;流速1.0mL/min;检测波长为238nm。结果表明,洛伐他汀和辛伐他汀在适当条件下可以完全水解成相应的开环酸。确定了洛伐他汀和辛伐他汀及有关物质的线性范围。本法快捷准确,可作为辛伐他汀原料药生产过程及洛伐他汀发酵液的质量控制方法。To investigate the hydrolysis of two lactone form statins, high performance liquid chromatographydiode array detection （HPLC-DAD） method was developed to determine Iovastatin and simvastatin and their related substances simultaneously. Waters Symmetry C18 column（4.6mm×150mm, 5μm） was used and the mobile phase was a mixture of acetonitrile-sodium dihydrogen phosphate （60：40）, flow rate 1.0mL/min, detected at 238nm. Under proper conditions, Iovastatin and simvastatin could be hydrolyzed to their corresponding β-hydroxy acids, respectively. The linear ranges of four related substances were determined by HPLC-DAD method.The method is simple, quick and accurate, can be used to the quality control of synthetic simvastatin as well as the lovastatin fermentation process.

关 键 词：HPLC-DAD 辛伐他汀 洛伐他汀 水解 含量测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]