蛇床子挥发油GC和GC-MS指纹图谱的研究  

Studies on Fingerprints of Fructus cnidii by GC and GC-MS

作　　者：赵陆华[1] 单臻[1] 李晓晶[1] 相秉仁[1] 

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室

出　　处：《中国药学杂志》2007年第12期889-891,共3页Chinese Pharmaceutical Journal

基　　金：国家"十五"重大科技专项"创新药物与中药现代化"基金资助项目(2002AA2Z3214)

摘　　要：目的采用GC和GC-MS色谱法研究并建立各产地蛇床子药材挥发油成分的指纹图谱。方法用DB-5石英毛细管柱(0.25 mm×30 m,0.25μm);以高纯氮为载气;流速1.0 mL.min-1;FID检测器。结果蛇床子药材指纹图谱选定了10个共有峰,峰面积之和大于总峰面积的90%,并且通过质谱进行了鉴别。结论本法重现性好,可结合蛇床子药材的HPLC指纹图谱评价其质量。OBJECTIVE To study the fingerprints of different Fructus cnidii samples by GC and GC-MS. METEODS The sep aration was performed on a DB -5（0. 25 mm×30 m,0. 25μm） column with the flow rate of 1.0 mL·min^-1. The carrier gas was nitrocen gas. The contents were determined by FID. RESULTS 10 Common peaks were obtained in the GC fingerprints of Fructus cnidii volatile oil and they were identified by GC-MS. CONCLUSION The method has good repeatability. This GC fingerprint combined with the HPLC fingerprint of Fructus cnidii can be used to evaluate its quality integrally.

关 键 词：蛇床子药材 挥发油 指纹图谱 气相色谱 气相色谱-质谱 

分 类 号：R284[医药卫生—中药学]