粒细胞集落刺激因子对脑卒中的神经保护作用研究  

Neuroprotection by Granulocyte-Colony Stimulating Factor (G-CSF) after focal cerebral ischemia.

作　　者：韩军良[1] 夏峰[1] 张光运[1] 赵刚[1] 

机构地区：[1]第四军医大学附属西京医院神经内科,710032

出　　处：《脑与神经疾病杂志》2007年第3期197-200,共4页Journal of Brain and Nervous Diseases

摘　　要：目的:研究在脑梗塞后不同时间窗内应用粒细胞集落刺激因子(G-CSF)的神经保护作用。方法:中风模型采用线栓MCA法,栓塞后90min后再灌注;动物采用雄性Wistar大鼠(n=45,体重280～320g)。实验组大鼠分别在中风后30min(n=11)、24h(n=12)、48h(n=10)开始皮下注射G-CSF(60ug/kg．day),至第7日结束;对照组大鼠(n=12)应用生理盐水0．3ml/日。每日检测大鼠体重变化。中风后7天,取脑进行形态学研究,分别进行anti-MPA2和TUNEL染色,计算脑梗塞体积和梗塞灶周围神经细胞凋亡情况。结果:对照组脑梗塞体积(163±25mm^3)明显大于30min(81±19mm^3)、24h(98±27mm^3)[P＜0．001]及48h(114±23mm^3)[P＜0．01]等各治疗组。对照组TUNEL阳性细胞数(187±21)明显高于MCAO后30min及24h开始应用G-CSF的治疗组(P＜0．01)。结论:G-CSF有明显的脑保护作用,即使在脑梗塞发生候后48小时才开始应用,效果仍然明显。Objective： To investigate the neuroprotective effects of Granulocyte colony-stimulating factor（G-CSF） when application at different time windows after ischemic stroke. Method： Wistar rats（n= 40, body weight 280-320 gram） were induced stroke by the filament model of a 90 min temporary middle cerebral artery occlusion （MCAO）. In treatment groups, annimals were randomized into 3 subgroutps according to the initial time point of application G-CSF after stroke onset： 30min（n= 10）, 24h（n=10） and 48h（n= 10）. Administration of G-CSF subcutaneous per day at a dosage of 60ug/ kg body weight was ceased on 7^th day after MCAO. In control group（n= 10）, each animal received saline of 0.3mi per day. Body weight of each animal was recorded each day. On 7h＇ day after MCAO, animals were killed and the brains were reserved for further histological investigations. Infarct volume and apoptosis was evaluated by anti-microtube protein-2 （MAP2） or TUNEI. staining respectively. Result.. Infarct size of control group（163＋25 mm^3） was significantly larger than that in each treatment group： 30min（81±19 mm^3, p〈0.001 ）, 24h（98±27 mm^3, p〈0.001 ） and 48h（114±23 mm^3 , p〈0.01）. Account of TUNEL positive cells aroud infarct area in control group（187＋21 ） was significantly larger than in groups application of G-CSF 30min and 24 hours after MCAO（p〈0.01）. Conclution： G-CSF intensively inhibited apoptosis, .even when administrated two day later but repeated injection for several days after MCAO, G-CSF still showed neuroprotective effects.

关 键 词：脑梗塞 神经保护 凋亡 G-CSF 

分 类 号：R743[医药卫生—神经病学与精神病学]