EEG和rCBF监测在大鼠非开颅SAH模型中的应用  

Application of EEG and rCBF in a non-craniotomy model of subarachnoid hemorrhage in rats

作　　者：金清东[1,2] 林秋泉[2] 陈志[1] 朱刚[1] 王宪荣[1] 吴国材[1] 杨慧[1] John.H.Zhang 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科,全军神经系统疾病微创诊治中心 [2]福建医科大学莆田市第一医院神经外科 [3]Division of Neurosurgery,Loma Linda University Medical Center,Loma Linda 92354,USA.

出　　处：《第三军医大学学报》2007年第13期1282-1284,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30500662)~~

摘　　要：目的了解头皮导联脑电图及局部脑血流量监测在制备大鼠非开颅SAH模型中的应用价值。方法SD大鼠48只,按随机数字表法分为正常对照组、假手术组和SAH组,参照Bederson方法建立大鼠非开颅SAH模型,对各实验组大鼠行头皮导联脑电图及局部脑血流量监测并观察大体病理解剖。结果各实验组术前脑电图和局部脑血流量无差异(P>0.05),正常对照组监测24h无明显变化;假手术组术后脑电图相对波幅轻微下降(8.48±2.01)%,5min后恢复达术前水平,局部脑血流量术后也轻度下降(7.60±0·60)%,10min后恢复达术前水平;SAH组大鼠术后1min内出现脑电图δ波增多,波幅显著下降,下降幅度达(77.86±3.25)%,24h脑电图与术前比较仍有差异(P<0.01),其下降幅度与蛛网膜下腔出血量及出血速度有关;SAH后局部脑血流量呈双相改变,出血后立刻下降,下降幅度为(78.00±2·39)%,10min时恢复为术前(89.85±5.72)%,随后又渐下降,2h后相对稳定在(52.41±2.72)%,并持续24h。结论头皮导联脑电图及局部脑血流量监测在大鼠非开颅血管内线穿刺法制备的SAH模型中可以有效判断是否成功穿刺血管,而且间接了解蛛网膜下腔出血的量与速度。Objective To evaluate electroencephalogram and regional cerebral blood flow （rCBF） in monitoring the establishment of a non-craniotomy model of subarachnoid hemorrhage （SAH） in rats. Methods The SAH model was established according to Bederson＇ s method. Forty-eight rats were randomly divided into control group, sham operated group and SAH group. The dynamic changes of EEG and rCBF as well as the pathological anatomy were observed in postoperative 24 h. Results EEG and rCBF had not diversity among three groups before operation and in the control group through the whole experiment. In the sham operated group, the amplitude of EEG decreased by （ 8.48 ±2.01 ） % in 5 min and rCBF also decreased by （7.60 ±0. 60 ） % in 10 min, then reverted to preoperative level. Abnormal observation was not found by pathological anatomy in the control and sham operated groups. The amplitude of EEG decreased by （77. 86 ±3.25 ） % and 8 wave increased in 1 min after SAH, and it at 24 h showed difference with the preoperative level. The decreased amplitude of EEG was correlated to subarachnoid hemorrhagic volume and velocity. The changes of rCBF had biphasic response, first decreased to （78.00 ± 2.39 ） % of the preoperative level immediately after SAH, and increased to （89.85 ± 5.72 ）% in 10 min, then decreased again to （47.68 ± 4.72）% within 2 h and lasted for 24 h stably. Conclusion The dynamic changes of EEG and rCBF can be taken to judge whether SAH is successfully established and indirectly reflect the volume and velocity of subarachnoid hemorrhage.

关 键 词：蛛网膜下腔出血 脑电图 局部脑血流量 

分 类 号：R-332[医药卫生] R741.044