产谷胱甘肽重组巴斯德毕赤氏酵母发酵条件的研究  

Optimization of Fermentation Conditions for Production of Glutathione in Recombinant Pichia pastoris

作　　者：饶志明[1] 艾丽静[1] 沈微[1] 方慧英[1] 诸葛健[1] 

机构地区：[1]江南大学工业微生物中心和工业生物技术教育部重点实验室

出　　处：《食品与发酵工业》2007年第5期1-4,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(30570142);江苏省青年科技创新人才(学术带头人)基金(BK2006504)资助项目

摘　　要：考察了培养基组成及培养条件对重组巴斯德毕赤氏酵母(Pichia pastoris)x-33(pGAPZA-gsh 1)合成谷胱甘肽(GSH)的影响。优化后的培养基组成为:甘油30g/L、蛋白胨40g/L、酵母膏9g/L、半胱氨酸0.36 g/L、KH_2PO_4 3g/L;培养条件为:自然pH、摇床转速200r/min、装液量为30mL/250mL、接种量为10%。在此优化条件下重组酵母的GSH产量是98.5mg/L,为优化前的2.33倍,生物量最大值达到19.6g/L。在5L发酵罐上进行放大实验,发酵结束后GSH产量、重组菌的生物量分别为97.9mg/L,18.7g/L与摇瓶发酵结果基本吻合。The medium composition and fermentation conditions for GSH producing in recombinant Pichia pastoris x-33 （pGAPZA- gsh 1） were optimized in the research. The maximal yield of GSH （98.5 mg/L） and the biomass （19. 6 g/L ） could be obtained when the recombinant strain was cultivated in the medium with concentrations of glycerol, yeast extract, tryptone, KH2PO4 and cysteine set at 30 g/L, 9 g/L, 40 g/L, 3 g/L and 0. 36 g/L, respectively, under the optimized cultivation conditions of natural pH, rotation speed 200 r/min and inoculation size 10 % in shake flask. The GSH production of the recombinant Pichia pastoris x-33 （pGAPZA- gsh 1） was 2.3 times that of the control. Meanwhile, the GSH yield （97.9 mg/L） and the biomass （18.7 g/L） were achieved when the recombinant strain was cultivated in a 5L fermenter using the above optimized fermentation conditions, which were similar with those of flask shake.

关 键 词：重组酵母 谷胱甘肽 摇瓶 发酵罐 

分 类 号：TQ929[轻工技术与工程—发酵工程]