胶质细胞源性神经营养因子对肌萎缩侧索硬化症培养模型的保护作用  

GDNF protect the model of amyotrophic lateral sclerosis

作　　者：肖向建[1] 刘卫刚[1] 李敏[2] 马征[2] 李春岩[2] 

机构地区：[1]河北省人民医院神经内科,石家庄050091 [2]河北医科大学第二医院神经内科

出　　处：《中国康复医学杂志》2007年第6期506-508,I0001,共4页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:利用肌萎缩侧索硬化症的脊髓片培养模型,观察胶质细胞源性神经营养因子(GDNF)对运动神经元的保护作用。方法:取出生后8天乳鼠的腰段脊髓组织切片做脊髓器官型培养,随机分为对照组、模型组、不同浓度GDNF组(1ng/ml、5ng/ml和50ng/ml),倒置显微镜观察脊髓片形态变化,SMI-32免疫组化观察前角运动神经元数目的变化。结果:对照组体外生长良好,而模型组生长不良,SMI-32阳性细胞较对照组减少,GDNF各组脊髓运动神经元存活数目较模型组显著增多,且具有量效关系。结论:GDNF能保护运动神经元免受谷氨酸兴奋毒性的损伤。Objective： Investigate the protective effects of glial cell derived neurotrophie factor （GDNF） on injured motor neurons mediated with glutamate. Method： Organotypie spinal cord cultures were made in 8-day-old rats. The slices were divided into 5 groups： control group, model group and GDNF 1ng/ml、5ng/ml and 50ng/ml group. We inspected the morphologie changes with inverted microscope and calculated the number of spinal motor neurons with SMI-32 immunohistoehemieal staining. Result： The spinal cord slices of GDNF groups had good morphology similar to those of the control group. The number of SMI-32 positive α motor neurons decreased in model group compared with control group while GDNF groups dose-dependently increased compared with model group. Conclusion： GDNF could protect motor neurons from the glutamate exeitotoxieity.

关 键 词：胶质细胞源性神经营养因子 肌萎缩侧索硬化 谷氨酸 脊髓 

分 类 号：R744[医药卫生—神经病学与精神病学]