紫菜多糖对小鼠TNF-α、NO的诱生作用  

Induction of Laver(Porphyra spp.) Polysaccharide on Mouse TFN-α and NO

作　　者：李胜军[1] 吕昌龙[1] 单风平[1] 李成[1] 曹妍[1] 闫建中[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2007年第3期47-48,共2页Journal of Microbiology

基　　金：辽宁省自然科学基金项目(20022065)

摘　　要：探讨紫菜多糖对小鼠免疫功能的刺激作用。以不同浓度的紫菜多糖及生理盐水(NS)经BALB/C小鼠腹腔连续注射7 d(1次/d)后,通过结晶紫染色法及Griess法分别测定小鼠的脾细胞分泌TNF-α、NO水平。0.15 g/kg紫菜多糖注射组小鼠脾细胞分泌TNF-α、NO的水平高于NS对照组(P<0.05)。结果可见,适宜浓度的紫菜多糖具有增强小鼠免疫功能的效应。In order to investigate the stimulation of laver （Porphyra spp. ） polysaccharide （LP） on mouse immunity, different concentration of LP and normal saline （NS） were injected into BALB/C mice intraperitoneally once a day, and seven days after the continuous injection, the levels of TFN-α and NO secreted by spleen cells of mice were measured through crystal violet staining assay and Griess reaction respectively. The results showed that the levels of TFN-α and NO secreted by spleen cells from mice injected with 0.15 g/kg LP were higher than those injected with NS alone （P 〈 0.05）. Therefore, proper concentration of LP possesses immunity reinforcement effects in mice.

关 键 词：紫菜多糖 TNF-Α NO 免疫调节 

分 类 号：R931.77[医药卫生—生药学] R967[医药卫生—药学]