肺腺癌bcl-2及p53表达意义探讨  

Study of the Expression of bBci-2 in Lung Adenocarcinoma

作　　者：于慧玲[1] 白晓燕[2] 李时荣[1] 夏养志[1] 

机构地区：[1]内蒙古医学院病理教研室,内蒙古呼和浩特010059 [2]南方医科大学病理教研室,广东广州510515

出　　处：《内蒙古医学杂志》2007年第5期520-522,共3页Inner Mongolia Medical Journal

摘　　要：目的：探讨肺腺癌原发灶及其淋巴结转移灶中凋亡相关基因bcl-2及p53的表达意义，为进行肺腺癌的分子生物学研究提供理论依据。方法：采用免疫组化S-P法，用抗bcl-2单克隆抗体及抗p53单克隆抗体分别对63例发生淋巴结转移的肺腺癌组织及其相应淋巴结进行标记分析，且按性别、年龄、分化程度、TNM分期等进行分组比较。结果：bcl-2与肺腺癌淋巴结转移、患者性别、年龄、分化程度无关（P〉0．05），TNM分期Ⅰ～Ⅱ期肺腺癌bcl-2阳性率大于Ⅲ～Ⅳ期肺腺癌，差异具有显著性（P〈0．05）；p53与肺腺癌淋巴结转移、患者性别、年龄无关（P〉0．05），与分化程度有关（P〈0．05），TNM分期肺腺癌p53阳性率Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期肺腺癌，差异具有显著性（P〈0．05）。结论：bcl-2及p53在肺腺癌发生发展过程中可能起一定作用．可作为评估肺腺癌预后的生物学指标。Objective：To investigate the significance of the expression of bcl - 2 and p53 in lung adenocarcinoma and lymph node metastasis. Method：The expression of bcl- 2 and p53 in 63 cases of lung adenocarcinoma with lymph node metastasis were analysed byimmunohistochemical SP method using monoclonal antibody to bcl- 2 and monoclonal antibody to p53. Results：bcl- 2 was not related to lymph node metastasis, patients＇ sex, age and differentiation in lung adenocarcinoma（P 〉 0.05）：the positive rate of bcl- 2 was higher in TNM stage Ⅰ～Ⅱ than in stage Ⅲ-Ⅳ （P〈 0.05） ;p53 was not related to lymph node metastasis, patients＇sex, age in lung adenocarcinoma（P〈 0.05）. p53 was correlated with differentiation（P （0.05）. The positve rate of p53 was higher in TNM stage Ⅲ～Ⅳ than in stage Ⅰ～Ⅱ （P〈0.05）. Conclusions：bcl- 2 and p53 may play role in the carcinogenesis of lung adenocarcinoma. They can be valuable in predicting the prognosis of lung adenocarcinoma.

关 键 词：肺腺癌 BCL-2 P53 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]