凋亡抑制基因Livin在食管癌中的表达及其与P53、Bcl-2的关系  

Expression of Livin in Esophageal Carcinoma and Its Correlation with P53,Bcl-2

作　　者：黄青远[1] 孙群利[2] 赵志龙[1] 崔肃[1] 陈东义[1] 张林[1] 

机构地区：[1]中国医科大学附属第一医院胸外科,沈阳110001 [2]黑龙江省医院胸外科,哈尔滨150001

出　　处：《中国胸心血管外科临床杂志》2007年第3期196-200,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：辽宁省教育厅基金资助项目(20122143)~~

摘　　要：目的探讨凋亡抑制基因Livin在食管癌组织中的表达及其与P53和Bcl-2表达的关系。方法采用逆转录-聚合酶链反应(RT-PCR)结合银染技术检测36例食管癌组织和18例癌旁正常组织中Livin mRNA的表达,采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶(S-P)法检测食管癌组织中Livin、P53和Bcl-2蛋白的表达。结果RT-PCR检测结果显示:Livin mRNA在食管癌组织中的阳性表达较癌旁正常组织明显增强,两种异构体基本同时表达。免疫组织化学检测结果显示:Livin蛋白在食管癌组织中的阳性表达率明显高于癌旁正常组织(P<0.01)。癌组织侵及食管外膜的Livin阳性表达率高于癌组织侵及食管肌层者(P<0.05);有淋巴结转移者的Livin阳性表达率高于无淋巴结转移者(P<0.05)。Livin蛋白表达与P53蛋白表达无关(χ2=1.00,P=0.505),与Bcl-2蛋白表达有关(χ2=10.60,P=0.003)。结论Livin在食管癌组织中异常表达,有望成为食管癌诊断和基因治疗的新靶点。凋亡相关基因Bcl-2与Livin基因的异常表达可能在食管癌癌变中起协调作用。Objective To investigate the expression of inhibitor of apoptosis gene Livin and its relationship with expression of P53,Bcl-2 in esophageal carcinoma tissues. Methods The expression of Livin messenger ribonucleic acid （mRNA） in 36 esophageal carcinoma tissues and 18 para-cancerous tissues were measured by reverse transcription-polymerase chain reaction （RT-PCR） combined with silver staining technique. The expression of Livin, P53 and Bcl-2 proteins were detected by immunohistochemical method （streptavidin-peroxidase）. Results RTPCR results： Livin mRNA positive expression of esophageal carcinoma tissues was more evident than that of paracancerous tissues, the expression of both variants was simultaneous basically. Immunohistochemical results： the Livin protein positive expression rate of esophageal carcinoma tissues was higher evidently than that of para-cancerous tissues （P〈 0. 01）. Livin protein positive expression rate of external coat of esophagus invaded by carcinoma was higher than that of tunica muscularis esophagi invaded by carcinoma（P〈0. 05）; Livin protein positive expression rate of lymph node metastasis was higher than that of normal lymph node （P〈0.05）. The expression of Livin protein was not related to the expression of P53 protein（X^2=1. 00,P=0. 505）,but it was positively related to the expression of Bcl-2 protein （X^2= 10. 60,P = 0. 003）. Conclusion Aberrant expression of Livin may be a new target for diagnosis and gene treatment of esophageal carcinoma. The aberrant expression of Livin and apoptosis related gene Bcl-2 may play synergetic roles in process of carcinogenesis of esophageal carcinoma.

关 键 词：LIVIN 食管癌 P53 BCL-2 

分 类 号：R735.1[医药卫生—肿瘤]