免疫毒素192-IgG-saporin致痴呆动物基底前脑一氧化氮合酶阳性神经元的变化  

Changes in NOS-positive neurons of basal forebrain in rat Alzheimer's model induced by immunotoxin 192-IgG-saporin

作　　者：罗秀梅[1] 龙大宏[1] 潘学兵[1] 杨丹迪[1] 冷水龙[1] 

机构地区：[1]广州医学院人体解剖学教研室,广州510182

出　　处：《解剖学杂志》2007年第3期265-268,共4页Chinese Journal of Anatomy

基　　金：广东省自然科学基金(04009566);广州市市属高校科技计划重点项目(041011);广东省医学科研基金(A2003276)

摘　　要：目的:建立侧脑室注射免疫毒素192-IgG-saporin致痴呆动物模型,观察其学习记忆能力和基底前脑一氧化氮合酶(NOS)阳性神经元的变化。方法:侧脑室注射免疫毒素192-IgG-saporin,行Y迷宫检测,采用NADPH-d组织化学法染色,结合图像分析技术观察大鼠基底前脑NOS阳性神经元数目和形态学参数的变化。结果:模型组大鼠的学习、记忆能力较正常组明显下降。模型组基底前脑内侧隔核(MS)和斜角带垂直支(VDB)NOS阳性神经元数分别减少到正常组的21.62%和17.70%;细胞周长和面积降低,灰度值升高,均有显著性差异。结论:应用192-IgG-saporin免疫毒素可使基底前脑NOS阳性神经元明显减少。Objective： To establish an animal model of Alzheimer＇s disease in rats and observe changes in learning and memory abilities and in NOS-positive neurons of the basal forebrain. Methods： 192-IgG-saporin was injected into the lateral ventricle of SD rats. Learning and memory abilities were tested by Y-maze. Morphologic data and percentages of NOS-positive neurons were measured in the basal forebrain by using NADPH-d histochemical method combined with image analysis. Results： The learning and memory abilities in the model group were sharply decreased, compared with the normal group. 21.62 % and 17.70 % of NOS-positive neurons respectively survived in medial septum （MS） and vertical diagonal band （VDB） of the basal forebrain in the model group. The area and perimeter of NOS-positive neurons in MS/VDB of the model group were significantly decreased. The gray value in MS/VDB of the model group was markedly increased. Conclusion： The number of NOS-positive neurons significantly decreases in the basal forebrain using immunotoxin 192-IgG-saporin in rat.

关 键 词：一氧化氮合酶 神经元 基底前脑 192-IgG-saporin 痴呆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]