神经生长因子与降钙素基因相关肽对局灶性脑缺血再灌注大鼠顶叶皮质环磷酸腺苷反应元件结合蛋白(CREB)和磷酸化CREB的上调作用  

NGF and CGRP up-regulate the expression of CREB and phospho-CREB during focal cerebral ischemia/reperfusion in rat parietal cortex

作　　者：张正洪[1] 曲鹏[2] 刘玉丽[2] 张宝辉[2] 方秀斌[2] 

机构地区：[1]郧阳医学院解剖学教研室,十堰442000 [2]中国医科大学基础医学院神经生物学教研室,沈阳110001

出　　处：《解剖学杂志》2007年第3期327-330,共4页Chinese Journal of Anatomy

基　　金：辽宁省自然科学基金(619019)

摘　　要：目的:探讨外源性神经生长因子(NGF)和降钙素基因相关肽(CGRP)对局灶性脑缺血再灌注大鼠顶叶皮质环磷酸腺苷反应元件结合蛋白(CREB)和磷酸化CREB(p-CREB)表达的影响。方法:用线栓法制作局灶性脑缺血再灌注模型,应用免疫组织化学SABC法、Western印迹法和图像分析方法检测大鼠手术侧顶叶皮质CREB和p-CREB表达。结果:缺血再灌注组手术侧顶叶皮质CREB表达较假手术组减少,p-CREB表达高于假手术组;NGF组和CGRP组顶叶皮质CREB和p-CREB表达均高于缺血再灌注组;NGF组和CGRP联合应用时CREB与p-CREB的表达更高。结论:NGF与CGRP对缺血神经元的保护作用可能是通过上调神经元内CREB和p-CREB来实现的,NGF和CGRP有协同作用。Objective： To investigate the effect of exogenous nerve growth factor （NGF） and calcitonin gene-related peptide （CGRP） on the expression of cyclic AMP response element-binding protein （CREB） and phosphorylatcd CREB （p-CREB） in rat parietal cortex during focal cerebral ischemia/reperfusion （I/R）. Methods： A focal cerebral ischemia/ reperfusion model was induced by occlusion of the right middle cerebral artery using an intraluminal suture method. Expressions of CREB and p-CREB in the right parietal cortex were detected using an immunohistochemical SABC method and Western blotting. The imrnunoreactive positive products were analyzed by image analysis system. Results： in the right parietal cortex the expression of CREB was reduced in I/R group compared with that in sham group, but the expression of p-CREB was higher in I/R group than that in the sham group （P〈0. 05） . The average absorbency （A） of CREB and p-CREB positive products in NGF group or CGRP group was higher than that in I/R group （P〈 0. 05）. Furthermore, the expression of CREB and p-CREB in NGF ＆ CGRP group was even higher than that in NGF or CGRP group （P 〈 0. 05）. Conclusion： CREB may be involved in the mechanism of protective effect of NGF and CGRP on ischemic neurons. NGF and CGRP might coordinate in this protective mechanism.

关 键 词：神经生长因子 降钙素基因相关肽 环磷酸腺苷反应元件结合蛋白 脑缺血 再灌注 

分 类 号：R363[医药卫生—病理学]