胡桃叶黄酮对D-半乳糖衰老模型小鼠的影响  

The Effect of Total Flavone of Juglans Regia Folium on Mice Doddery Models Created by D-galactose

作　　者：郑奕辉[1] 李荔[1] 张奕鹏[1] 庄丽贤[1] 

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041

出　　处：《国际医药卫生导报》2007年第12期4-8,共5页International Medicine and Health Guidance News

基　　金：广东省汕头市重点科技计划项目（编号：2005-116）

摘　　要：目的 观察胡桃叶总黄酮对d-半乳糖衰老模型小鼠的SOD、ACHE、MDA的影响,探讨其抗衰老作用及机制。方法 小鼠随机均分为6组,除正常对照组外,各组每天皮下注射D-半乳糖（0.05g/kg）,连续注射6周。从注射的第7天开始,3个实验组同时灌胃高、中、低剂量的胡桃叶总黄酮,阳性对照组灌以维生素E（0.05g/kg）模型对照组与正常对照组每天灌胃等量的蒸馏水,连续给药5周后测定血清SOD、ACHE和脑组织MDA的含量变化。结果 实验组的血清SOD显著高于模型对照组（P〈0.05）,ACHE活力极显著高于模型对照组（P〈0.01）,实验组的脑MDA含量显著低于模型对照组（P〈0.05）;与正常对照组比,各项指标相近（P〉0.05）。结论 胡桃叶总黄酮能提高抗氧化能力,降低自由基含量,增强胆碱能神经系统功能,从而防止衰老。Objective To observe the effects oftotal flavone on superoxide dismutase （SOD）, ACHE , malondiadehyde （MDA） in effects and mechanism of Total flavone of Juglans regia of Juglans regia folium mice to study therapeutic folium on doddery models created by D-galactose. Method Mice were randomly divided into 6 groups. All groups, except the control, were administrated （ip） with D-galactose （0. 05g/kg） for successive 6 weeks. The 3 experimental groups were administrated （ig） with high, middle and low dosages oftotal flavone of Juglans regia folium respectively after 7th day of the injection of D-galactose whereas vitamin-E at 0. 05g/kg body weight served as a positive control. The model and control groups were daily adminisered with D.D.W. for continuous 5 weeks. After treatments, the SOD, ACHE activity in serum and MDA in brain of rats were determined. Result The serum SOD, ACHE activities in total flavone of Juglans regia folium -treated mice were higher than that in control group. However, the brain MDA content in total flavone of Juglans regia folium-treated mice was lower than that in control group. Conclusion This data suggested thattotal flavone of Juglans regia folium had a significant anti-oxidative effect by reducing the free radicals as well as an anti-aging effect through the enhancement of cholinergic nerve system.

关 键 词：D-半乳糖 胡桃叶总黄酮 衰老 小鼠 

分 类 号：R-332[医药卫生]