大豆荚壳中类黄酮提取工艺及其抑杀线虫活性的研究  

STUDIES ON THE EXTRACTION TECHNOLOGICAL CONDITIONS OF TOTAL FLAVONOIDS IN SOYBEAN HULL AND ITS NEMATICIDAL ACTIVITY

作　　者：赵卫星[1] 孙治强[1] 高玉红[2] 张楠[1] 

机构地区：[1]河南农业大学林学园艺学院,郑州450002 [2]郑州职业技术学院,郑州450121

出　　处：《大豆科学》2007年第3期373-376,共4页Soybean Science

基　　金：河南省重大科技攻关项目(0322010900)

摘　　要：阐述从大豆荚壳中提取类黄酮的工艺及其抑杀根结线虫的应用效果。通过正交设计,确定了大豆类黄酮的最佳提取工艺条件为乙醇浓度80%,提取温度70℃,乙醇与大豆荚壳1:10,回流提取24 h,提取率可达8.58%,显著高于次佳工艺。对根结线虫抑杀效果的测定表明:类黄酮粗提物对南方根结线虫J2有很强的毒杀效果,且随使用浓度增大而增强,1.5μg.mg-1类黄酮提取物处理,线虫的校正死亡率可达50%以上;对虫卵孵化有一定的抑制作用,但孵化抑制率都在50%以下。The optimal extracting technological conditions of flavonoids from soybean hull by orthogonal plan method and its nematicidal activity were studied. The result indicated that the conditions of 70℃, 10：1 of 80% ethanol to soybean hull,heating and refluxing for 24 h were optimium, the extractive ratio could be up to 8.58 % and was higher than Hypo-optimal conditions significantly;The total flavonoids of the extractive solution had high nematicidal activity against the second juvenile（J2）, and with the in creasing of concentration, the nematicidal activity was improved,more than 50% of J2 could be killed with the treatment of 1.5/μ ·mg^-1 total flavonoids extraction. In addition,it also had inhibition on eggs hatch to some extent, but inhibition rate of eggs hatch was below 50%.

关 键 词：大豆荚壳 类黄酮 提取工艺 杀线虫活性 

分 类 号：TQ28[化学工程—有机化工]