幽门螺杆菌尿素酶B亚单位Th表位的免疫学特性研究  

Characterization of the Th epitopes in the urease B subunit of Helicobacter pylori.

作　　者：石云[1] 吴超[1] 周维英[1] 邹全明[1] 

机构地区：[1]第三军医大学临床微生物及免疫学教研室暨重庆市生物制药技术中心,重庆400038

出　　处：《现代免疫学》2007年第3期177-181,共5页Current Immunology

基　　金：国家自然科学基金(30400406)

摘　　要：采用MHC限制性分析、ELISA方法、淋巴细胞增殖实验等方法研究幽门螺杆菌(Hp)尿素酶B亚单位(UreB)的H- 2~d限制性Th表位U_(546-561)、U_(229-244)、U_(237-251)的免疫学特性。发现抗I-E^d抗体能够抑制U_(546-561)对CD4^+T淋巴细胞的刺激,抗I-A^d抗体能够抑制U_(229-244)、U_(237-251)对CD4^+T淋巴细胞的刺激作用。U_(229-244)、U_(229-244)能刺激CD4^+T淋巴细胞分泌IL-4和IL-10,U_(237-251)刺激CD4^+T淋巴细胞分泌IFN-γ、IL-2,且U_(546-561)、U_(229-244)、U_(237-251)三个表位肽免疫BALB/c小鼠能够引起针对各自免疫多肽和rUreB的CD4^+T细胞应答。结果表明U_(546-561)为I-E^d限制性Th2表位,U_(229-244)为I-A^d限制性Th2表位、U_(237-251)为I-A^d限制性Th1表位。三个表位之间具有协同刺激效应,可以用于Hp表位疫苗的研究。The immulogical characteristcs of the H-2^d restricted Th epitopes in the urease B subunit of Helicobacter pylori was clarified by means of MHC-restriction, ELISA and lymphocyte proliferation assay. It was demonstrated that the stimulatory effects of epitope U546-561 and epitopes U229-244 and U237-251 to CD4^＋ T lymphocytes could be inhibited by anti-I-E^d antibody and I-Ad antibody respectively. The epitopes U606-661 and U225-244 could enhance the production of IL-4 and IL-10 by CD4^＋ T lymphocytes, while epitope U237-251 could stimulate the secretion of IFN-y and IL-2 by CD4^＋ T lymphocytes. In addition, immunization of BALB/c mice with epitopes U546-541 , U229-244 and U232-251 could induce the CD4^＋ T cell responses against the corresponding peptides used for immunization and recombinant urease B subunit （rUreB）. These results indicate that U546-561 represents the I-E^d restricted Th2 type epitope, U229-244 represents the I-A^d restricted Th2 type epitope , and U237-251 was the I-A^d restricted Thl type epitope. There was synergistic interaction among these 3 epitopes and they can be used for vaccine design.

关 键 词：幽门螺杆菌 尿素酶B亚单位 TH表位 BALB/c(H-2d)小鼠 

分 类 号：R392.11[医药卫生—免疫学]