BMP-7在SD大鼠肝脏的表达  

Expression of BMP-7 in Liver Tissue of SD Rat

作　　者：丁芹[1] 崇雨田[1] 马会慧[1] 杨绍基[1] 

机构地区：[1]中山大学附属第三医院感染科

出　　处：《中国误诊学杂志》2007年第12期2692-2694,共3页Chinese Journal of Misdiagnostics

基　　金：本课题获国家自然科学基金资助项目(编号:30571665)

摘　　要：目的:通过对正常SD大鼠及肝功能衰竭模型、肝纤维化模型肝组织中骨形态发生蛋白7(bone morpho-genetic protein-7,BMP-7)表达的分析,初步探讨BMP-7在肝脏炎症和肝细胞再生过程中可能发挥的作用。方法:分别采用腹腔注射四氯化碳或D-氨基半乳糖的方法构建SD大鼠肝纤维化模型及肝功能衰竭模型。采用RT-PCR方法研究BMP-7 mRNA的表达,并对4例正常SD大鼠、11例肝功能衰竭模型、3例肝纤维化模型中BMP-7 mRNA的表达进行实时荧光定量RT-PCR分析。结果:BMP-7在正常SD大鼠肝脏有表达,BMP-7/β-actin在正常大鼠肝组织(0.449±0.154)高于肝功能衰竭模型肝组织(0.044±0.024),低于肝纤维化模型肝组织(3.174±2.525),与后者的差异无显著性。结论:BMP-7在正常SD大鼠肝脏有表达,在肝纤维化模型表达增加,在肝功能衰竭模型表达减少。Objective：To investigate the possible effects of bone morphogenetic protein 7 （bone morphogenetie proteins,BMP-7） on liver inflammation and hepatoeyte regeneration by analysis of its expression in the liver of normal SD rat, model of hepatic fibrosis and model of hepatic failure. Methods：SD rat models with hepatic fibrosis were made through intraperitoneal injection of 40% carbon tetraehloride 0.2 ml per 100 g rat body weight three times perweek for six weeks while models with hepatic failure was obtained 24 hours after intraperitoneal injection of D-galaetosamine 800 1 200 mg per 1 000 g rat body weight. To study the expression in the liver tissues of normal and model SD rat by RT- PCR and gel eleetrophoresis. BMP 7 mRNA expression in the liver of 4 normal SD rat, 3 SD rat models with hepatic fibrosis and 11 SD rat models with hepatic failure was quantitated by real-time RT-PCR. Results： BMP-7 mRNA expressed in the liver of SD rat, BMP-7/β-actin in the liver tissue of normal SD rat （0. 449±0. 154） was higher than that in hepatic failure models（0. 044±0. 024） while lower than that in the hepatic fibrosis models（3. 174±2. 525）, but the difference with the latter is of no statistical significance. Conclusion：BMP7 expression in the liver of normal SD rat, increases in hepatic fibrosis model, but decreases in hepatic failure model.

关 键 词：骨形态发生蛋白质类/生物合成 肝/代谢 肝功能衰竭/代谢 肝硬化/代谢 

分 类 号：R575[医药卫生—消化系统]