马巴贝斯虫PCR检测方法的建立及应用  

Establishment and application of the PCR method for detection of Babesia equi

作　　者：熊焕章[1] 刘冰[1] 张守发[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《延边大学农学学报》2007年第2期129-133,共5页Agricultural Science Journal of Yanbian University

摘　　要：根据已发表的马巴贝斯虫18S rRNA基因序列,设计并合成一对特异性引物,通过聚合酶链式反应(PCR)扩增出一条约936 bp的基因片段,并成功地将该基因克隆于pGEM-Teasy载体,经PCR鉴定后进行序列测定.结果表明,所克隆的目的基因与GenBank中发表的序列同源性达到98.4%.并利用特异性引物初步建立了马巴贝斯虫病PCR检测技术.A pair of specific primers were designed and synthesized by published 18s rRNA gene sequence of Babesia equi. A 936bp gene fragment was obtained by PCR and cloned into the pGEM- T easy vector. The fragment was sequenced after identified with PCR. The sequence data demonstrated that the homology of nucleotide sequence was 98.4 % compared with that of Genbank. PCR assay for identification of Babesia equi was developed by specific primers.

关 键 词：马巴贝斯虫 18S RRNA PCR检测 

分 类 号：S858.23[农业科学—临床兽医学]