黄鳝生殖干细胞在性逆转生殖发育过程中的变化  

TRANSFORMATION OF THE GERM STEM CELLS IN THE GONAD DEVELOPMENT OF SEX REVERSAL IN MONOPTERUS ALBUS

作　　者：肖亚梅[1] 陈丽莉[1] 陈松[1] 刘姣[1] 赵如榕[1] 

机构地区：[1]湖南师范大学生命科学学院教育部蛋白质化学和鱼类发育生物学重点实验室,长沙410081

出　　处：《分子细胞生物学报》2007年第3期196-204,共9页Journal of Molecular Cell Biology

基　　金：湖南省自然科学基金项目资助(05JJ30070);湖南省教育厅科研基金项目资助(02C219)。

摘　　要：应用组织学方法及免疫组织化学技术显示,黄鳝性逆转生殖发育过程中,生殖干细胞(GSCs)定位分布于生殖褶中,黄鳝雌性发育阶段的GSCs分散或成团存在,间性及雄性发育阶段GSCs均区分为A、B两种不同类型,雌性发育阶段GSCs与A、B两类GSCs在超微结构上存在差异。结果表明,生殖褶中GSCs是黄鳝分化生殖腺中唯一具有有丝分裂能力的生殖细胞群,雌性发育阶段GSCs表现出卵原干细胞特征,间性及雄性发育阶段GSCs为精原干细胞。CD49整合素是黄鳝雌性发育阶段GSCs和A类GSCs的表征分子。This paper investigates the features of GSCs in the process of the gonad development of sex reversal in Monopterus a/bus by the histological methods and the histological immunochemical techniques. In Monopterus a/bus,the GSCs are located in the gonadal lamellae,which are scattered or accumulated during the female phase. In the intersex and the male,the GSCs are distinguished by two types A and B, both of them differ from the GSCs at the female stage in ultrastructure. It shows that the GSCs existing in the gonadal lamellae are the unique germ family, which owns the capability of mitosis in the differential gonads. The GSCs represent the ovogonia in the female phase,while the spermagonia in the intersex and the male phases. CD49 is the molecular marker for the GSCs at the female stage and the GSCs of type A.

关 键 词：黄鳝 性逆转 生殖干细胞 生殖质 CD49 

分 类 号：Q954.4[生物学—动物学]