流动注射-化学发光法测定氢化可的松  

Determination of hydrocortisone by flow-injection chemiluminescence

作　　者：熊迅宇[1] 唐玉海[1] 王楠楠[1] 武世界[2] 

机构地区：[1]西安交通大学分析科学研究所,陕西西安710061 [2]兰州大学化学系,甘肃兰州730000

出　　处：《西安交通大学学报（医学版）》2007年第3期340-342,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的确定以高锰酸钾-亚硫酸钠体系测定氢化可的松的流动注射-化学发光分析方法。方法在酸性条件下,氢化可的松对高锰酸钾-亚硫酸钠体系发光反应具有明显的增敏作用。据此,建立了流动注射化学发光测定氢化可的松的分析方法。结果在优化的实验条件下,氢化可的松质量浓度在1.0×10-9-1.0×10-6g/mL范围内与发光强度呈良好的线性关系,检出限(3R)为4.0×10-10g/mL,对氢化可的松进行11次平行测定,其相对标准偏差为2.2%。结论本方法应用于注射液中氢化可的松含量的测定,快速、准确、简便,灵敏度高、线性范围宽。Objective To establish a new flow-injection chemiluminescence （CL） method for the determination of hydrocortisone. Methods In H2SO4 solution, hydrocortisone could obviously enhance the chemiluminescence intensity of the reaction of KMnO4-Na2SO3 system. Based on this, a new flow-injection CL method for the determination of hydrocortisone was developed. Results There was a good linear relationship between CL intensity and the concentration of hydrocortisone in the range of 1.0 × 10^-9 -- 1.0 × 10^-6g/mL. The detection limit was 4.0 ×10^-10g/mL at a signal-to-noise ratio of 3 ： 1. The RSD of 11 assays was 2.2%, Oonclusion This method can be successfully used to determine the quantity of hydrocortisone in injection, It is rapid, accurate, simple, and has high sensitivity and wide linear range.

关 键 词：流动注射 化学发光 氢化可的松 

分 类 号：R914.1[医药卫生—药物化学]