2种昆虫高活性抗菌肽制备方法的优化  

Optimization of Antibacterial Peptides Preparation from Tenebrio molitor and Spodoptera exigua

作　　者：索相敏[1] 陆秀君[1] 董建臻[1] 李静[1] 李瑞军[1] 刘顺[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001

出　　处：《华北农学报》2007年第3期119-122,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金项目(C200700450)

摘　　要：以黄粉虫（Tenebrio molitor）幼虫和甜菜夜蛾（Spodoptera exiguaHuber）幼虫为材料,比较了针刺蘸大肠杆菌、针刺蘸高效氯氰菊酯、紫外线照射、饥饿等诱导方式对抗菌肽活性的影响,并对其提取方法及特性进行了研究。结果表明,几种处理方式均能诱导2种昆虫产生抗菌肽,以针刺蘸大肠杆菌法得到的抗菌肽的抑菌活性最强,其次是针刺蘸高效氯氰菊酯法,饥饿处理法的活性最弱。用1 mol/L乙酸铵溶解抗菌肽抑菌活性提高;在pH 5-9的条件下处理抗菌肽对其活性影响不大;反复冻融活性下降。The effects of different inducing methods on the activity of antibacterial peptides from Tenebrio molitor and Spodostera exigua Huber were studied in this paper. The larvae were treated by pricking with Escherichia coli, pricking with β-cypermephrin, radiating with ultra-light and starving. The results showed that the antibacterial peptides could be induced from Tenebrio molitor and Spodoptera exigua Huber by these methods. The activity of antibacterial peptides from pricking with Escherichia coli was the best, The activity was stronger than pricking with β-cyperme phrin. The activity of antibacterial peptides was the lowest by starving the larvae. The activity was higher treated by 1 mol/L acetic-ammonia. In the pH value range of five to nine, the activity of antibacterial peptides was almost the same. And it＇s activity descend when treated with icy-hot treat.

关 键 词：黄粉虫 甜菜夜蛾 抗菌肽 制备方法优化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]