皮肤成纤维细胞纤维连接蛋白及其EDA、EDB表达与损伤时间的关系  

The Relationship Between Fibronectin EDA/EDB mRNA in Skin Fibroblast and Injury Time

作　　者：余松[1] 薛爱民[2] 贺盟[2] 赵子琴[2] 沈忆文[2] 顾云菊[2] 

机构地区：[1]湖州市公安局,浙江湖州313000 [2]复旦大学上海医学院法医学系,上海200032

出　　处：《法医学杂志》2007年第3期188-190,共3页Journal of Forensic Medicine

基　　金：浙江省自然科学基金资助项目(200333052110)

摘　　要：目的研究培养的皮肤成纤维细胞纤维连接蛋白(fibronectin,FN)及其可变剪接片段EDA、EDB在损伤后的mRNA水平,为推断早期损伤时间提供参考指标。方法培养人体皮肤成纤维细胞,换无血清培养液培养24h后,采用划痕法制作细胞损伤模型,损伤后0、0.5、1、2、6、24h收集细胞,抽提RNA,实时RT-PCR定量检测总FN及其EDA、EDB的mRNA水平的变化。结果皮肤成纤维细胞中总FN及其EDA、EDB的mRNA水平随损伤时间的延长而升高,EDA mRNA在损伤后1h达到高峰,EDB mRNA在损伤后6h达到高峰,FN mRNA在损伤后2h达到高峰;在损伤1h后总FN及其EDA、EDB的mRNA表达变化随时间的延长而呈现一种不断上升的趋势。结论纤维连接蛋白EDA、EDB可作为早期损伤时间推断的较理想的参考指标。Objective In order to effectively estimate early injury time, The transcription of fibronectin and ahernative splicing segment of fibronectin-EDA/EDB were studied. Methods The human skin fibroblast cells were cultured and incubated in serum-free medium for 24 h. The cells were injured with scratch technique. The cells were harvested at 0, 0.5, 1, 2, 6 and 24 h after injuries. Total RNA was extracted and real-time RT-PCR was pefformed to quantify mRNAs for fibronectin, EDA and EDB. Results The mRNA level of fibronectin, EDA and EDB were increased after injury and showed a gradually increased tendency. The EDA mRNA peaked in lh after injury, EDB mRNA 6h, fibronectin mRNA 2h. Conclusion FN-EDA/EDB may be used as a sensitive marker for the estimation of early injury time. K

关 键 词：成纤维细胞 纤维连接蛋白 EDA EDB 早期损伤时间推断 实时RT-PCR 

分 类 号：R734.2[医药卫生—肿瘤]