益肠泰对人大肠癌细胞株CCL229细胞增殖和酶活性的抑制作用  

The Inhibitory Effect of Yichangtai on Cell Proliferation and Enzyme Activity in Human Colon Cancer Cell CCL229

作　　者：张丽红[1] 陈晓琳[1] 王旭[1] 蔡玉文[1] 

机构地区：[1]辽宁中医药大学组织胚胎学教研室,辽宁沈阳110032

出　　处：《解剖科学进展》2007年第2期155-157,共3页Progress of Anatomical Sciences

基　　金：辽宁省教育厅基金资助(05L255)

摘　　要：目的研究中药复方益肠泰对人大肠癌细胞株CCL229 Mg2+-ATP酶,G-6-P酶活性的影响,探讨其抑制大肠癌细胞增殖的抗癌机制。方法采用血清药理学方法,制备药物血清。应用MTT法测定细胞增殖抑制率,通过酶细胞化学方法观察Mg2+-ATP酶,G-6-P酶活性的变化。结果药物血清作用CCL299细胞株24h,中药小剂量组未见明显抑制作用,其余各组均表现出不同程度的抑制作用,中药大剂量组抑制率达54.78%。药物血清作用CCL299细胞株48h,中药小剂量组出现抑制作用,各药物血清组随着作用时间的延长抑制更明显,中药大剂量组抑制率达65.60%,抑制效应呈时间依赖性。药物血清作用48h,Mg2+-ATP酶、G-6-P酶活性明显受到抑制。结论益肠泰具有抑制人大肠癌细胞株增殖的作用,降低Mg2+-ATP酶,G-6-P酶的活性是益肠泰抑制大肠癌细胞增殖的重要抗癌机制之一。Objective To investigate the effect of Yichangtai on enzyme activities of Mg^2＋ -ATPase and G-6- Pase in human colon cancer cell CCL229. Methods Serum pharmacological method was used for culturing cells, using MTT assay to observe the effect of Yichangtai on proliferation of human colon cancer cell . The effect of Yichangtai on cell enzyme activity was detected by enzyme cytochemical method. Results Yichangtai containing serum has evident inhibitory effect on the human colon cancer cell line CCL229 with time dependence.The inhibiting rate of Yichangtai on CCL229 cells is 54.78% for 24h culture, 65.60% for 48h serum culture . Enzyme cytochemistry results show that the activities of G-6- Pase and Mg2^＋-ATPase in the colon are decreased by Yichangtai. Conclusion Yichangtai can inhibit the proliferation of the human colon cancer cell CCL229, decreasing the enzyme activities of G-6-Pase and Mg2^＋-ATPase, which may be one of its mechanism.

关 键 词：益肠泰 大肠癌 血清药理学 酶细胞化学 

分 类 号：R735.34[医药卫生—肿瘤]