兔扩张型心肌病模型的实验研究  

Experimental Study on Rabbit Dilated Cardiomyopathic Model

作　　者：金波[1] 施海明[1] 朱军[1] 罗心平[1] 黄成磊[1] 刘兴党[2] 王逸平[3] 刘从进[2] 

机构地区：[1]复旦大学附属华山医院心内科,上海200040 [2]复旦大学附属华山医院核医学科,上海200040 [3]中科院上海药物研究所,上海201203

出　　处：《实验动物与比较医学》2007年第2期77-80,共4页Laboratory Animal and Comparative Medicine

基　　金：上海市卫生局科研课题项目(沪卫教2005-37)

摘　　要：目的经耳缘静脉注射盐酸阿霉素复制新西兰兔扩张型心肌病模型,应用光镜、电镜、心肌核素显像和血流动力学指标测定评价该模型。方法普通级雄性新西兰兔20只,随机分为对照组和模型组,经耳缘静脉注射盐酸阿霉素建立DCM模型,血流动力学指标测定评价心功能改变;99mTc-MIBI心肌灌注显像明确对照组和模型组心肌灌注的差异;光镜观察心肌组织形态学特点;透射电镜观察心肌组织超微结构的改变。结果与对照组比较,血流动力学各项指标改变均具有统计学意义;心肌核素显像示模型组心室壁变薄,心腔扩大,心尖部灌注减低;心肌组织形态学和超微结构的改变亦符合扩张型心肌病的特征。结论兔阿霉素心肌病模型能够较好地模拟临床病理生理过程,为干细胞移植治疗DCM的基础研究提供理想的动物模型。Objective To establish Adriamycin-induced dilated cardiomyopathic（DCM） model through ear-border vein in New Zealand rabbit, and the model were evaluted by light microscopy,electron microscopy, myocardial perfusion imaging and indexes of hemodynamics. Methods Twenty male New Zealand rabbits were randomly divided into model group and control group equally. Adriamycin was injected to produce DCM model through ear-border vein. Indexes of hemodynamics were measured to evalute the cardiac function.^99mTc-MIBI myocardial perfusion imaging showed the difference between control group and model group.Histomorphological changes were evaluted by light microscopy and ultrastructure characteristics were investigated by transmission electron microscopy. Results Cardiac examinations indicated there was statistical significance compared with control group. Myocardial perfusion imaging indicated low infusion in cardiac apex, ventricular cavity became enlarged and the wall was thin.The histomophological and ultrastructure changes ofmyocardium coincide with the characteristics of DCM. Conclusions The adriamycin-induced cardiomyopathic rabbit model was similar to the histopathological changes of DCM in humans,it may be used for stem cell basic research in cardiology.

关 键 词：扩张型心肌病 心力衰竭 新西兰兔 动物模型 ^99MTC-MIBI 

分 类 号：R-332[医药卫生]