外周血单核细胞激活效应在原发性高血压患者丹参酮干预过程中的反应(英文)  

Inhibitory effect of tanshinone on the activation of peripheral blood mononuclear cells in patients with essential hypertension

作　　者：占成业[1] 陶秀良[2] 周代星[1] 郑智[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北省武汉市430030 [2]华中科技大学同济医学院附属同济医院中医药研究室,湖北省武汉市430030

出　　处：《中国组织工程研究与临床康复》2007年第24期4828-4832,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省科技攻关资助(2003AA301C47)~~

摘　　要：背景:外周血单核细胞预激活是动脉粥样硬化形成的重要早期事件和促进因素之一,丹参酮是从传统中药丹参中提取的一种脂溶性成分,具有确切的抗动脉粥样硬化作用。目的:通过检测外周血单核细胞的黏附活性与分泌活性,分析原发性高血压患者病程早期是否存在外周血单核细胞预激活,并探讨丹参酮对体外培养条件下外周血单核细胞激活的抑制作用。设计:病例对照分析。单位:华中科技大学同济医学院附属同济医院急诊科与中医药研究室。对象:选取2003-01/2004-10华中科技大学同济医学院附属同济医院心血管内科收治的未经治疗或已停用降压药物2周以上的原发性高血压患者30例,男16例,女14例;平均年龄(44.6±7.4)岁;体质量指数(26.2±4.5)kg/m2;平均病程(38.5±16.9)个月;对本实验均知情同意。根据1999年WHO/ISH的高血压诊断标准,均为高血压Ⅰ～Ⅱ级。排除继发性高血压、器质性心脏病、高脂血症、糖尿病、肝肾功能障碍、感染等临床情况以及高血压所致的心、脑、肾、血管等靶器官损害。另选择30例血压正常的健康体检者作为正常对照组。人脐静脉内皮细胞(武汉大学物种保存中心);丹参酮注射液(雅安三九药业有限公司,批号020724)。方法:①入选者均抽取静脉血4.0～5.0mL,采用Ficoll-Hypaque密度梯度离心法和塑料吸附法分离单个核细胞,37℃孵育40～60min后收集贴壁细胞即为外周血单核细胞。瑞氏染色后镜下观察细胞形态符合单核细胞特征,锥虫蓝拒染试验测定活细胞数>95%。将新鲜分离的外周血单核细胞重悬,按4×107 L-1接种到24孔培养板上,每份标本设3孔:第1孔为基础分泌孔,第2孔为血管紧张素Ⅱ刺激孔,第3孔为丹参酮预处理孔。在血管紧张素Ⅱ刺激前先将外周血单核细胞与丹参酮共同孵育30min。血管紧张素Ⅱ、丹参酮终浓度分别为1×10-8mol/L和1×10-8g/L,外周血单核�BACKGROUND： Preactivation of peripheral blood mononuclear cells （PBMCS） is one of the most important eerly events and facilitating factor for the formation of atherosclerosis. Tanshinone is a lipolytic component extracted from traditional Chinese medicine of denshen, it has definite anti-atherosclerotic effect.OBJECTTVE： To analyze whether PBMCS preactivation existed at early essential hypertension, and investigate the effects of tanshinone on inhibiting the PBMCS activation cultured in vitro by detecting the adhesion and excretory activities of PBMCS.DESTGN： A case-controlled analysis.SETTING： Department of Emergency and Research Room of Traditional Chinese Medicine, Tongji Hospital affiliated to Tongji Medical College, Huazhong University of Science and Technology.PARTTCTPANTS： Thirty patients with untreated essential hypertension or with withdrawal from antihypertensives for at least 2 weeks were selected from the Department of Cardiology, Tongji Hospital affiliated to Tongji Medical College,Huazhong University of Science and Technology from January 2003 to October 2004, including 16 males and 14 females, aged （44.6±7.4） years, body mass index of （26.2±4.5） kg/m^2, average disease course of （38.5±16.9） months.Informed contents were obtained from all the subjects. Their hypertension was grade Ⅰ-Ⅱ according to the diagnostic standards for hypertension by WHO/ISH in 1999. Secondary hypertension, organic heart disease, hyperglyceridemia,diabetes mellitus, liver and kidney dysfunction, heart, brain, kidney, vessel and other target damaged induced by infection and other clinical conditions and hypertension were excluded by history, physical examination and assistant examination.Another 30 healthy physical examinees with normal blood pressure were enrolled as the normal control group. Human umbilical vein endothelial cells （Species Reserving Center of Wuhan University）; Tanshinone injection （Yaan Sanjiu Pharmaceutical, Co., Ltd., batch number： 020724）;METHODS： ① Venous b

关 键 词：单核细胞 预激活 丹参酮 高血压/遗传学 冠状动脉硬化 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]