三七总皂苷对大鼠肠缺血再灌注损伤的保护作用  

Preventive Effects of Panax Notoginsenoside Against Intestine I-R Injury in Rats

作　　者：钱忠义[1] 黄东[2] 沈志强[3] 

机构地区：[1]昆明医学院病理教研室,云南昆明650031 [2]昆明医学院第一附属医院检验科,云南昆明650032 [3]昆明医学院云南省天然药物药理重点实验室,云南昆明650031

出　　处：《昆明医学院学报》2007年第3期13-16,共4页Journal of Kunming Medical College

基　　金：云南省自然科学基金资助(2004C0044M);云南省医药与生物技术创新人才联合培养基地资助(YNCX2003)

摘　　要：目的探索三七总皂苷对大鼠小肠缺血再灌注损伤的保护作用.方法采用肠系膜上动脉夹闭-松夹方式复制大鼠小肠缺血再灌注损伤模型.实验动物分4组,即模型对照组(缺血再灌注组)、三七总皂苷200mg/kg和400mg/kg实验组、假手术组.实验组在建立肠缺血再灌注损伤模型12h前灌胃给予三七总皂苷预处理.分别采用鲨试验偶氮显色法和酶联紫外分光光度法检测血浆LPS和D-乳酸.取肝、脾、肠系膜淋巴结及血液分别作细菌培养.取回肠一段,按Chiu氏分级法进行病理组织学的分类评价.结果模型对照组细菌培养阳性数显著增加,而实验组的明显减少.两种剂量的三七总皂苷均明显降低血浆LPS浓度,400mg/kg的三七总皂苷降低血浆D-乳酸浓度,而200mg/kg剂量则无明显影响.病理形态学检查结果显示,三七总皂苷明显改善缺血再灌注引起小肠的病理性改变.结论三七总皂苷对小肠缺血再灌注损伤有较好保护作用.Objective To study the effect of Panax notoginsenoside on intestinal ischemia -reperfusion （I - R） injury in SD rats. Methods The I - R rat model was prepared by occluding the superior mesenteric artery （SMA） for 1 h, and followed by reperfusion for 1 h. SD rats were divided into four groups： intestinal ischemia - reperfusion group, Panax notoginsenoside - pretreated two groups （200 mg/kg and 400 mg/kg）, and Sham - operated group. D -lactate and endotoxin （lipopolysaccharide, LPS） levels were determined, and the I -R intestine changes were examined by histology. Results The levels of LPS and D -lactate in Panax notoginsenoside -pretreated groups were lower as compared with control group. The bacterium positive rates in Panax notoginsenoside - pretreated groups were lower than control group. Intestine histological change in control group was more significant than that in Panax notoginsenoside - pretreated groups. Conclusion Panax notoginsenoside showed marked preventive effects against intestine I -R injury in rats.

关 键 词：小肠 缺血再灌注 三七总皂苷 

分 类 号：R574[医药卫生—消化系统]