神经干单细胞获得的一种方法  

A method to obtain neural stem single cells

作　　者：曾飞跃[1] 周仁辉[1] 黄永凯[1] 罗湘颖[1] 方芳[1] 卢明[1] 陈成[1] 

机构地区：[1]中南大学湘雅医院神经外科,湖南长沙410008

出　　处：《中国医学工程》2007年第4期327-329,共3页China Medical Engineering

摘　　要：目的寻找一种有效获得神经干细胞单细胞的方法。方法运用胰蛋白酶,EDTA和不同浓度酵素对人胚神经干细胞球进行消化,免疫细胞化学染色鉴定及其分化。结果运用一定浓度的酵素可以获得大量成活的单个神经干细胞。而运用胰蛋白酶,EDTA消化后成活细胞少。结论运用一定浓度的diapase消化神经干细胞球是可获得大量成活的神经干细胞单个细胞的方法。[Objective] To investigate an effective way to isolate neural stem cells sphere（neurospheres）. [Methods] .Neurospheres were dissociated by digestion with trypsin, EDTA and different dose of dispase, and immunohistochemistry techniques were used for the neurospheres and their differentiation. [Result] Lots of viably single neural stem cells can be obtained by a certain of dispase, then the neurospheres with trypsin and EDTA were damaged and few viably cells obtained. [Conclusion] It＇s an ideal mothod by using dispase to digest neurosphere for single neural stem cell.

关 键 词：酵素 神经干细胞球 单细胞 

分 类 号：Q2-33[生物学—细胞生物学]