龙眼体细胞胚胎发生过程中特异表达蛋白的双向电泳分析  

2-DE analysis of the proteins expressed specifically during somatic embryogenesis in longan

作　　者：安娜[1] 赖钟雄[1] 郭志雄[2] 

机构地区：[1]福建农林大学园艺植物生物工程研究所 [2]福建农林大学园艺学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2007年第3期244-249,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30471204);福建省自然科学基金资助项目(C0410012);福建省科技厅重大资助项目(2004NZ02-2)

摘　　要：以龙眼品种红核子LC2胚性细胞系为材料,进行体细胞胚胎(简称体胚)发生及其同步化调控,获取体胚发生过程中各个主要发育阶段的培养物(胚性愈伤组织、球形胚、子叶形胚、早期成熟胚、中期成熟胚、晚期成熟胚),采用双向电泳对体胚发生过程中特异表达蛋白进行分析.结果表明:(1)龙眼胚性愈伤组织中,pI为4-5的酸性蛋白表达种类最多,在体胚发育的过程中逐渐消失,相反,pI为5-6的微酸性蛋白的表达逐渐增多;(2)体胚发育后期,尤其是成熟胚晚期,分子质量大的蛋白大量减少,分子质量较小的蛋白增多并逐渐积累;(3)虽然在各个发育阶段都会出现一些新的特异蛋白,但是没有改变蛋白质种类逐渐减少的趋势;(4)发现了一些有差异的特异蛋白点,尤其是成熟胚后期,有一系列大分子质量的蛋白消失,新出现了一部分分子质量较小的蛋白,其中几种表达量很大,可能与体胚发育有密切关系.In this experiment, the LC2 embryogenic cell line of the cultivar Honghezi of longan （ Dimocarpus longan Lout. ） was used as materials for obtaining the synchronized embryogenic cultures of somatic embryogenesis at different developmental stages （the embryogenic callus, globular embryo, cotyledonary embryo, early-stage mature embryo, mid-stage mature embryo and latestage mature embryo） for further 2-DE analysis of the proteins expressed specifically during somatic embryogenesis in longan. The 2-DE analysis showed that ： （ 1 ） the embryogenic callus of longan expressed the most varieties of acidic proteins （ the values of pI were between 4 and 5 ）, which eliminated gradually in the process of development of somatic embryos, on the opposite, slightly acidic proteins （ the values of pI were between 5 and 6） increased gradually; （2） during the late developmental stages, the high-molecular-weight （HMW） proteins eliminated gradually, and the low-molecular-weight （LMW） proteins simultaneously appeared and accumulated gradually; （3） although there were specific spots at each stage, the general diversificational trend was obviously decreased gradually; （4） there were some differential spots standing for specific proteins, especially at the late-stage somatic embryogenesis, a series of HMW proteins disappeared, and a series of LMW proteins appeared, which included some highly expressed proteins that were probably closely associated with somatic embryogenesis.

关 键 词：龙眼 体细胞胚胎发生 特异表达蛋白 双向电泳 

分 类 号：S667.2[农业科学—果树学] Q786[农业科学—园艺学]