凤尾侧耳多糖的分离纯化及抗氧化作用  

The Isolation, Purification, Determination of Pleurotus Membrancens Polysaccharide and Its Antioxygenation

作　　者：许燕萍[1] 笪远峰[1] 杨伟珠 陈琪[2] 

机构地区：[1]镇江医学院,镇江212001 [2]南京医科大学动脉粥样硬化研究中心

出　　处：《中国生化药物杂志》1997年第2期59-61,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金

摘　　要：凤尾侧耳子实体经热水提取，Sevag法去蛋白，流水透析、乙醇沉淀、丙酮洗涤得灰白色粉末状物质（凤尾侧耳多糖）。经水解后纸层析鉴定为单一斑点，与半乳糖比移值相同，均为产Rf=0．26。比旋光度[α]26D=+33°，紫外光谱分析不含蛋白质和核酸。红外光谱和核磁共振谱分析显示该多精含β-糖苷键。体外实验表明，凤尾侧耳多糖对人血浆低密度脂蛋白（LDL）氧化反应有明显的抑制作用，提示凤尾侧耳多糖具有抗氧化能力。Water-soluble grey-white powder polysaccharide from pleurotus membrancens was isolated with hot water and purified with further processes. Its hydrolysate was showed one spot by P. C. And the Rf was 0. 26, equal to that of galactose. The purified polysaccharide was without protein and nucle ic, [α]D26= +33°. The pleurotus membrancens polysaccharide showed characteristic absorbance in IR spectrum and 1H-NMR spectrum indicating the presence of the β-configuration. Pleurotus membran cens polysaccharide inhibited human LDL from oxidized significantly in vitro tests indicating its an tioxygenation.

关 键 词：凤尾侧耳 多糖 提取 抗氧化 药理 

分 类 号：R914.4[医药卫生—药物化学] TQ464.1[医药卫生—药学]