马铃薯环腐病菌快速检测方法(NCM-ELISA)的建立  

Establishment of NCM-ELISA for Rapid Test of Potato Ring Rot

作　　者：胡林双[1] 王晓丹[1] 闵凡祥[1] 郭梅[1] 李学湛[1] 白艳菊[1] 

机构地区：[1]黑龙江省农业科学院植物脱毒苗木研究所农业部脱毒马铃薯种薯质量监督检验测试中心(哈尔滨),黑龙江哈尔滨150086

出　　处：《中国马铃薯》2007年第3期142-145,共4页Chinese Potato Journal

基　　金：黑龙江省自然科学基金项目(2004C0307)

摘　　要：建立检测马铃薯环腐病菌NCM-ELISA方法并组装成试剂盒,应用于检测、检疫和流行病学调查具有实践意义。本研究以马铃薯环腐病菌为抗原,制备兔抗血清,利用碱性磷酸酯酶标记的羊抗兔血清(GAR-AP)为酶标二抗,建立了马铃薯环腐病菌NCM-ELISA快速检测方法。结果表明:抗血清的最佳工作浓度为1:400,最低检出菌液浓度为1.0×106个.mL-1,特异性也比较强。因此,该方法具有敏感性高、特异性强、速度快、实用方便等特点,可以应用于马铃薯环腐病菌的快速检测和诊断。Establishment of NCM-ELISA and assembly of the technique into kits are helpful for testing, quarantine and epidemiology study of potato ring rot. Antiserum was prepared by rabbits with the antigen of potato ring rot, and GAR-AP was enzyme-linked secondary antibody. The results indicated that the suitable working concentration was 1：400, and the lowest testing concentration of bacterium liquid was 1.0×10^6 bac · mL^-1. The NCM-ELISA has many advantages, such as high sensitivity, better specialty, time saving, practicality and convenience, and therefore can be used for rapid testing and diagnosis of potato ring rot.

关 键 词：马铃薯 环腐病 ELISA NCM-ELISA 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]