方格星虫酶解物成分分析及其抗氧化作用  

The Nutritional Components of Enzymatic Hydrolysate from Sipunculus nudus and Effects on Anti-oxidation

作　　者：张桂和[1] 赵谋明[1] 巫光宏[2] 

机构地区：[1]华南理工大学轻工与食品学院 [2]华南农业大学生命科学学院,广州广东广州510642

出　　处：《食品与生物技术学报》2007年第3期80-84,共5页Journal of Food Science and Biotechnology

基　　金：广东省科技计划项目(2003B21304);广州市科技攻关项目(200421-E0061)

摘　　要：用木瓜蛋白酶对方格星虫(Sipunculus nudus)进行酶促水解,并对酶解物冻干粉的成分进行了分析,其中粗蛋白质量分数为69.02%,多肽质量分数为60.60%,肽的平均链长为3.73个氨基酸残基,肽分子的平均相对分子质量为447.6;游离氨基酸占5.27%。氨基酸分析结果显示,方格星虫酶解物含有17种氨基酸(色氨酸未测),其中人体必需氨基酸含量较高,占总氨基酸数的30.62%。方格星虫酶解物中还含有P、Fe、Mg、Mn、Zn、Cu、Se等至少12种矿质元素,以及含有抗氧化作用的营养成分,并通过动物试验证明了方格星虫酶解物具有显著的抗氧化作用。方格星虫酶解物可用于食品添加或用作功能性食品。The nutritional components in Sipunculus nudus after being partly hydrolyzed by papain was systematically investigated. The results showed that the enzymatic hydrolysate lyophilized powder riched in oligo-peptide（average 3.73 amino acid residue）, mineral elements（P,Fe, Mg, Mn, Zn, Cu, Se, and so on） and all the essential amino acids. The hydrolysis products of Sipunculus nudus with an enzymatic hydrolysis degree of 26.79% contained about 60. 60% of oligo-peptides which average molecular weight was 447.6 Dalton. To study the anti-oxidative effect of the enzymatic hydrolysate of Sipunculus nudus ,the hemolysis extent of red blood cells was detected by spectrophotometric methods,and self-oxidative inhibition was studied using the 1,2,3-phentriol. The results demonstrated that the enzymatic hydrolysate of Sipunculus nudus had evident antioxidation effects. It may be the source for functional food.

关 键 词：方格星虫 酶解物 成分分析 抗氧化作用 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]