巨噬细胞移动抑制因子对大肠癌细胞侵袭的影响  

Impact of macrophage migration inhibitory factor on cell migration of colorectal carcinoma cell lines in vitro

作　　者：李晓宇[1] 何兴祥[1] 

机构地区：[1]广州医学院第二附属医院消化科,510260

出　　处：《现代临床医学生物工程学杂志》2006年第5期402-404,共3页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然科学基金(30470435);教育部留学回国人员科研启动基金(教外司留[2003]406号);广东省自然科学基金(031766);广东省科学技术厅科技计划项目(2004B31201015);广州市教育局科技计划项目(1025)

摘　　要：目的研究巨噬细胞移动抑制因子(MIF)在体外对大肠癌细胞侵袭能力的影响。方法大肠癌细胞株Lovo在外源性MIF刺激24h后用微孔迁移法检测通过微孔细胞数量的改变。RT-PCR检测基质金属蛋白酶2、9(MMP-2、MMP-9)和血管内皮生长因子(VEGF)mRNA表达情况。流式细胞术测定自发凋亡率变化。结果(1)MIF刺激后Lovo通过8μm微孔的细胞数增加[(174±9)个比(262±19)个,P=0.002)]。(2)VEGF121,VEGF165和MMP-9mRNA的表达增加(P<0.05)。MMP-2mRNA的表达无明显变化(P=0.616)。(3)Lovo自发凋亡率明显降低(P<0.001)。结论MIF能够抑制体外大肠癌细胞的凋亡,并能提高Lovo的体外侵袭能力。Objective To determine the influence of macrophage migration inhibitory factor （MIF） on the invasion of colorectal carcinoma cell （CC） lines, Lovo, in vitro. Methods The invasion of CC cell lines ,Lovo ,was evaluated by micron-migration assay in a chamber with 8 μm porosity polycarbonate filter membrane. RT-PCR was adopted to evaluate the mRNA level of matrix metalloproteinase-2 ,-9 （ MMP-2 ,-9 ） and vascular endothelial growth factor （ VEGF ）. The change of apoptosis rate of CC cell lines was measured by flow cytometry. Results （ 1 ） The number of cells passing through filter membrane was significantly increased（ 174 ± 9 vs 262 ± 19, P= 0. 002）. （2） VEGF121, VEGF165 and MMP-9 mRNA increased significantly（ P 〈 0.05 ）. No apparent difference of MMP-2 mRNA expression （ P = 0. 616） was observed. （ 3 ） The apoptosis rate of cells treated by MIF was reduced from 6. 2% ± 0. 45% to 2. 2% ±0. 50%. Conclusions MIF can inhibit the apoptosis of CC cell and increase cell invasion of CC cell lines in vitro. Mild up-regulated MMP-9 and VEGF by MIF may be possibly involved in the invasion of CC cell lines.

关 键 词：巨噬细胞移动抑制因子 结直肠肿瘤 肿瘤浸润 

分 类 号：R735.34[医药卫生—肿瘤]