基质金属蛋白酶2，9及其抑制剂3在脑梗死中的作用  

Effect of the matrix metalloproteinases-2, matrix metalloproteinases-9 and tissue inhibitor metalloproteinases-3 in the rats with cerebral infraction caused by injection of auto blood thrombus

作　　者：万林[1] 杜立峰[1] 孙师元[1] 

机构地区：[1]西安交通大学医学院第二附属医院急诊科,西安710004

出　　处：《中华急诊医学杂志》2007年第6期611-614,共4页Chinese Journal of Emergency Medicine

摘　　要：目的通过注入自体血栓形成大鼠脑梗死模型,研究其不同时段基质金属蛋白酶(matrix metalloproteinase,MMP)2、9和基质金属蛋白酶抑制剂(tissue inhibitor metalloproteinase, TIMP)3的表达规律。方法66只成年Sprague Dawley大鼠随机分为3组,空白对照组(6只)、假手术对照组(30只)和手术实验组(30只)。实验组给予自体血栓经颈内动脉注入制备大鼠脑梗死标本;假手术对照组做同样处理,但不给予血栓注入干预,于术后24 h,48 h,3 d,5 d,7 d麻醉并灌注固定取脑。观察大鼠行为学改变;采用免疫组化方法检测标本MMP-2,MMP-9,TIMP-3表达,了解其与病变之间关系。结果实验组大鼠行为学改变较其他组明显;HE染色下,组织病理变化明显;免疫组化染色显示MMP-2,MMP-9,TIMP-3表达均较其他两组增高,统计学结果显著性差异(P<0.05)。结论MMP-2和MMP-9在脑梗死中与疾病损伤相关,TIMP-3有对抗作用。Objective To investigate the effect of the cerebral infarction in rats and the role of matrix metalloproteinases-2 （MMP-2）, matrix metalloproteinases-9 （MMP-9）, and tissue inhibitor of metalloproteinases-3 （TIMP-3） in the pathogenesis. Method Sixty-six rats were randomly divided into 3 groups： blank group （ n = 6）, control group （ n = 30）, model group （ n = 30）. The rat model was established by carotid injection of auto blood thrombus, the control group was given the operation without injection. Each group was subdivided into 24 hours, 48 hours, 3 days, 5 days, 7 days after operative intervention. At serial intervals, the animals in each group were sacrificed and brain tissue samples were collected to determine MMP-2, MMP-9, TIMP-3 expression by immunohistochemistry. The changes of behaviors were observed and the histopathological changes were investigated by hematoxylin and eosin stain. Results The experimental group had more obvious behavior change than other groups. Hematoxylin and eosin stain showed significant histopathological changes; MMP-2, MMP-9 and TIMP-3 expression was up-regulated in comparison with other groups （ P 〈 0.05）. Conclusions The higher expression of MMP-2 and MMP-9 were devived from the injury and brain dysfunction in cerebral infraction. TIMP-3 inhibited the effect of the MMP-2 and MMP-9 activation.

关 键 词：基质金属蛋白酶 基质金属蛋白酶抑制剂 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]