深层发酵生产灵芝菌丝体多糖研究  

Study on the Production of Polysaccharide from Ganoderma lucidum Mycelia in Deep-liquid Fermentation

作　　者：魏赛金[1] 涂晓嵘[1] 黄林[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西科学》2007年第3期292-294,301,共4页Jiangxi Science

基　　金：江西省科学技术厅2004年度江西省重点新产品计划(编号:20041C1012900)

摘　　要：采用生物工程发酵技术进行灵芝菌730菌丝体多糖扩大生产,在500 L全自动不锈钢发酵罐中通过控制深层发酵工艺条件获得灵芝菌丝体、灵芝多糖。结果表明:发酵70 h,镜检菌丝壁上无明显的芽头和锁状联合,菌丝体有少许自溶,无杂菌。灵芝菌丝体的浓度达到30%左右,胞外灵芝多糖和胞内灵芝多糖分别达3.5g/L和4.8%。Through biological engineering fermentation technology, making full use of the high productivity of deep - liquid fermentation, to produce G. lucidum 730 myeelia and polysaccharide in 500 L automatic stainless steel fermenter. The results were as follows, after a fermention time of 70 hours, there was no obvious vgerminate and lock like connection on the mycelia wall. There was a little mycelia dissolved with no other bacteria. The consistence of G. lucidum clump is about 30% ,Extracellular Ganoderma Polysaccharide and Intracellular Ganoderma Polysaccharide is 3.5 g/L and 4.8% respectively.

关 键 词：灵芝菌丝体 灵芝多糖 发酵 

分 类 号：Q949.3[生物学—植物学]