hELP3 Subunit of the Elongator Complex Regulates the Transcription of HSP70 Gene in Human Cells  

作　　者：Qiuju HAN Xiaozhe HOU Dongmei SU Lina PAN Jizhou DUAN Liguo CUI Baiqu HUANG Jun LU 

机构地区：[1]Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第6期453-461,共9页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （No. 30370316）；the National Basic Research Program of China （2005CB522404）；the Program for Changjiang Scholars and Innovative Research Team （PCSIRT） in Universities （IRT0519）

摘　　要：The human Elongator complex is remarkably similar to its yeast counterpart in several aspects. In a previous study, we analyzed the functions of the human elongation protein 3 （hELP3） subunit of the human Elongator by using an in vivo yeast complementation system. However, direct evidence for hELP3 functions in regulating gene expression in human cells was not obtained. In this study, we used hELP3 antisense oligonucleotide inhibitors to knock down hELP3 gene expression to investigate its function in human 293T cells. The results showed that specific reduction of hELP3 mRNA and protein caused a significant suppression of HSP70-2 gene expression, and this was accompanied by histone H3 hypoacetylation and decreased RNA polymerase Ⅱ density at the HSP70-2 gene. Moreover, the data also showed that hELP3 exerted the transcriptional regulatory function directly through its presence on the HSP70-2 gene. Data presented in this report provide further insight and direct evidence of the functions of hELP3 in：HSP70-2 gene transcriptional elongation in human cells.The human Elongator complex is remarkably similar to its yeast counterpart in several aspects. In a previous study, we analyzed the functions of the human elongation protein 3 （hELP3） subunit of the human Elongator by using an in vivo yeast complementation system. However, direct evidence for hELP3 functions in regulating gene expression in human cells was not obtained. In this study, we used hELP3 antisense oligonucleotide inhibitors to knock down hELP3 gene expression to investigate its function in human 293T cells. The results showed that specific reduction of hELP3 mRNA and protein caused a significant suppression of HSP70-2 gene expression, and this was accompanied by histone H3 hypoacetylation and decreased RNA polymerase Ⅱ density at the HSP70-2 gene. Moreover, the data also showed that hELP3 exerted the transcriptional regulatory function directly through its presence on the HSP70-2 gene. Data presented in this report provide further insight and direct evidence of the functions of hELP3 in：HSP70-2 gene transcriptional elongation in human cells.

关 键 词：hELP3 histone acetylation transcription elongation ANTISENSE 

分 类 号：Q5[生物学—生物化学]